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Article
Arabidopsis kinome: after the casting
Arabidopsis thaliana is used as a favourite experimental organism for many aspects of plant biology. We capitalized on the recently available Arabidopsis genome sequence and predicted proteome, to draw up a geno...
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Article
AtSKθ, a plant homologue of SGG/GSK-3 marks develo** tissues in Arabidopsis thaliana
The Arabidopsis thaliana AtSK sub-family of serine threonine protein kinases groups 10 homologues of SHAGGY/GSK-3. Previous results obtained with different plant members of the SHAGGY/GSK-3 family strongly sugges...
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Article
Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana
The higher plant Arabidopsis thaliana (Arabidopsis) is an important model for identifying plant genes and determining their function. To assist biological investigations and to define chromosome structure, a coor...
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Article
Status of manual medicine in the emergency ward of an orthopaedic university clinic
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Article
Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana
The plant Arabidopsis thaliana (Arabidopsis) has become an important model species for the study of many aspects of plant biology1. The relatively small size of the nuclear genome and the availability of extensiv...
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Article
Nanoscale atomic lithography with a cesium atomic beam
line (852 nm) and a width of about 120 nm and covering a large area of approximately 1 mm2.
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Article
Pattern generation with cesium atomic beams at nanometer scales
We have demonstrated that a cesium atomic beam can be used to pattern a gold surface using a self assembling monolayer (SAM) as a resist. A 12.5 μm period mesh was used as a proximity mask for the atomic beam....
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Chapter
Are Arrestin-Like Proteins Involved in Plant Signal Transduction Pathways?
Arrestin molecules represent a class of proteins involved in controlling different transduction pathways mediated by G-protein related receptors. Visual arrestin (or S-antigen) is a 48 kDa soluble protein of r...
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Article
Gene density and organization in a small region of the Arabidopsis thaliana genome
We have characterized a 6.4 kb genomic fragment from Arabidopsis thaliana ecotype Columbia overlap** the 5′ end of the AKin10 gene which encodes a protein Ser/Thr kinase. Using, as probes, various restriction f...
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Chapter
Improvement of Barley and Wheat Quality by Genetic Engineering
Although the mature cereal grain consists predominately of starch with only about 10–15% of protein, it is the protein fraction which is largely responsible for quality. In the case of wheat the quality for br...
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Article
Spatial distribution of β-amylase in germinating barley seeds based on the avidin-biotin-peroxidase complex method
In comparison with two wild type barley cultivars, Sundance and Bomi, biochemical data show that the high-lysine mutant Hiproly contains abundant amounts of lysine-rich β-amylase, whereas mutant Risø 1508, als...
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Chapter
Transient Gene Expression in Wheat (Triticum aestivum) Protoplasts
Recent advances in methods of genetic transformation of plants have resulted in the introduction, stable integration, and heritable transmission of foreign genes in a number of species. However, the economical...
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Article
A method for large-scale progeny screening of putative transformed cereal
A simple large-scale screen is presented for the selection of transformed cereals and for progeny testing of transformants.
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Article
Multiple molecular forms of β-amylase in seeds and vegetative tissues of barley
The molecular forms of β-amylase present in develo**, mature, germinating and malted grains of barley (Hordeum vulgare L.), and in vegetative tissues, have been studied using Western-blot analyses and isoelectr...
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Article
Isolation, culture and morphogenesis from wheat protoplasts, and study of expression of DNA constructs by direct gene transfer
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Chapter
Isolation, Culture and Morphogenesis from Wheat Protoplasts, and Study of Expression of DNA Constructs by Direct Gene Transfer
The regeneration of plants from protoplasts of some monocots has been achieved, but so far the list excludes wheat. We report here on the culture of protoplasts obtained from an inflorescence-derived suspensio...
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Article
Location of β-amylase sequences in wheat and its relatives
A β-amylase cDNA clone isolated from barley has been used to locate β-amylase encoding sequences on wheat, rye, and Aegilops umbellulata chromosomes by hybridisation to restriction endonuclease digested DNA obtai...
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Chapter
Improvement of the Processing Properties of British Crops by Genetic Engineering
Two events have resulted in an unprecedented change in the face of UK agriculture over the past 20 years (see Table 1). The first was the passing of the Plant Varieties Act in 1964. This enabled plant breeders...
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Article
Polymorphism at the Hor 1 locus of barley (Hordeum vulgare L.)
The Hor 1 locus of barley encodes a group of seed storage polypeptides called C hordein. Two-dimensional electrophoretic analysis of C-hordein fractions from six cultivars with different alleles at the Hor 1 locu...
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Article
Molecular analysis of the effects of the lys 3a gene on the expression of Hor loci in develo** endosperms of barley (Hordeum vulgare L.)
The lys 3a gene present in the barley mutant Risø 1508 results in an increased content of lysine in the grain. Previous studies have shown that this increase results from a decreased accumulation of hordein and a...
