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Article
Irish Thoracic Society
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Article
Immunological detection of nitric oxide synthase(s) in human tissues using heterologous antibodies suggesting different isoforms
Nitric oxide (NO) is generated from l-arginine by NO synthases. Localization of the brain enzyme has been carried out in the rat; however, despite data suggesting that NO is a major regulator of vascular and neur...
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Article
Immunohistochemical measurements of nerves and neuropeptides in diabetic skin: relationship to tests of neurological function
Image-analysis was used to measure nerves immunoreactive to the general neuronal marker protein gene product 9.5 (PGP 9.5-IR) and the neuropeptides calcitonin gene-related peptide and vasoactive intestinal pol...
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Article
CGRP-immunoreactive endocrine cell proliferation in normal and hypoxic rat lung studied by immunocytochemical detection of incorporation of 5′-bromodeoxyuridine
We have tested the suggestion that the reported increase, in hypoxic rats, in the number of lung endocrine cells immunoreactive for the regulatory peptide CGRP is caused by an accumulation of peptide within th...
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Chapter
Endothelin, a Ubiquitous Peptide: Morphological Demonstration of Immunoreactive and Synthetic Sites and Receptors in the Respiratory Tract and Central Nervous System
After the original description of endothelin by Yanagisawa and colleagues68 in 1988 and the subsequent demonstration of the endothelin family,29 much attention has been devoted to the actions and effects of the p...
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Article
Spatial distribution of β-amylase in germinating barley seeds based on the avidin-biotin-peroxidase complex method
In comparison with two wild type barley cultivars, Sundance and Bomi, biochemical data show that the high-lysine mutant Hiproly contains abundant amounts of lysine-rich β-amylase, whereas mutant Risø 1508, als...
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Chapter
The Morphological Localization of Cholecystokinin and Its Binding Sites in the Diffuse Neuroendocrine System
Cholecystokinin (CCK) clearly belongs to the group of substances known as brain-gut peptides; it functions both as a neuropeptide and a gut hormone. A major hindrance to the investigation of CCK localization h...
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Article
Peptidergic nerves in human dental pulp
The peptidergic innervation of human dental pulp was studied with indirect immunofluorescence and immunoperoxidase techniques. Pulpal nerve fibres displaying immunoreactivity for cholecystokinin, calcitonin ge...
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Article
Early increase in CGRP- and VIP-immunoreactive nerves in the skin of streptozotocin-induced diabetic rats
We have previously shown depletion of nerves and neuropeptides in skin biopsies of diabetic patients, even in the absence of clinical signs and symptoms of sensory and autonomic neuropathy, but were unable to ...
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Article
Non-isotopic RNA probes
Several studies have shown the use of non-radioactive labelled DNA probes for in situ hybridisation, mainly to identify cellular DNA. In this study mRNA in situ hybridisation was performed on rat pituitary wit...
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Article
Neuron-specific enolase and its mRNA are highly expressed in large congenital nevi: A study using immunocytochemistry, biochemical assay, and in situ hybridization
The malignant transformation of congenital nevocellular nevi, both large and small, is controversial and presents problems in management. The size of the lesion is taken to indicate potential malignant transfo...
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Article
Depletion of cutaneous nerves and neuropeptides in diabetes mellitus: an immunocytochemical study
Immunocytochemistry for the general neuronal marker protein gene product 9.5 and four neuropeptides (calcitonin gene-related peptide, substance P, vasoactive intestinal polypeptide and neuropeptide Y) was perf...
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Article
Neuron-specific enolase and its mRNA are highly expressed in large congenital nevi: A study using immunocytochemistry, biochemical assay, and in situ hybridization
The malignant transformation of congenital nevocellular nevi, both large and small, is controversial and presents problems in management. The size of the lesion is taken to indicate potential malignant transfo...
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Article
Localisation of mRNA and co-expression and molecular forms of GRP gene products in endocrine cells of fetal human lung
The presence of bombesin (gastrin-releasing peptide, GRP)-like immunoreactivity in mucosal endocrine cells of human fetal lung is well established. In this study we have investigated the localisation of pro-GR...
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Article
Expression of the C-terminal peptide of human pro-bombesin in 361 lung endocrine tumours, a reliable marker and possible prognostic indicator for small cell carcinoma
Small cell carcinoma of the lung is a highly malignant tumour. Its known biological products which include bombesin, do not allow the prediction of tumour behaviour. Molecular biology has revealed the amino ac...
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Article
Antibodies to neurofilament protein and other brain proteins reveal the innervation of peripheral organs
Monoclonal and polyclonal antibodies to neurofilament proteins, neuron-specific enolase, glial fibrillary acidic protein and S-100 have been used to demonstrate nerves, ganglion cells and the supportive glial ...
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Article
The potential of the immunogold-silver staining method for paraffin sections
The immunogold-silver staining technique is shown to be of great value in the detection of regulatory peptide-containing nerves and endocrine cells in routinely fixed, paraffin-wax-embedded tissues. The method...
