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Article
Enhanced microbial lipid production by Cryptococcus albidus in the high-cell-density continuous cultivation with membrane cell recycling and two-stage nutrient limitation
As a potential feedstock for biofuel production, a high-cell-density continuous culture for the lipid production by Cryptococcus albidus was investigated in this study. The influences of dilution rates in the sin...
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Article
Lipid production by microalgae Chlorella protothecoides with volatile fatty acids (VFAs) as carbon sources in heterotrophic cultivation and its economic assessment
Volatile fatty acids (VFAs) that can be derived from food wastes were used for microbial lipid production by Chlorella protothecoides in heterotrophic cultures. The usage of VFAs as carbon sources for lipid accum...
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Article
Thermal Properties and Biodegradability Studies of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
For investigating the relationship between thermal properties and biodegradability of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), several films of PHBV containing different polyhydroxyvalerate (HV) frac...
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Article
Exploring low-cost carbon sources for microbial lipids production by fed-batch cultivation of Cryptococcus albidus
Volatile fatty acids (VFAs), acetic acid, acetates, and ethanol were used as carbon sources for the production of microbial lipids using Cryptococcus albidus in batch cultures. C. albidus utilized organic acids l...
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Article
Economic evaluation of off-gas recycle pressure swing adsorption (PSA) in industrial scale poly(3-hydroxybutyrate) fermentation
The use of high purity oxygen as a feeding gas in microbial fermentation has recent gained huge attention in industrial scale process due to its advantageous effect to increase viable cell density as well as t...
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Article
Production of biomass and recombinant human-like collagen in Escherichia coli processes with different CO2 pulses
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Article
Production of biomass and recombinant human-like collagen in Escherichia coli processes with different CO2 pulses
The evolution of CO2 in a fed-batch culture of recombinant Escherichia coli containing human-like collagen (HLC) cDNA was determined with an O2-enriched air supply (40%, v/v) in a 12.8 l fermentor; a maximum CO2 ...
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Article
Mass production of medium-chain-length poly(3-hydroxyalkanoates) from hydrolyzed corn oil by fed-batch culture of Pseudomonas putida
A novel biopolyester, Medium-Chain-Length Polyhydroxyalkanoates (MCL-PHAs), was made from the corn oil hydrolysate, an inexpensive and renewable carbon source, by a fed-batch culture of Pseudomonas putida with a ...
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Article
Modeling of poly(3-hydroxybutyrate) production by high cell density fed-batch culture of Ralstonia eutropha
High cell density culturing has been conducted for the production of poly(3-hydroxybutyrate) fed-batch cultures ofRalstonia eutropha with phosphate limitation. It was found that a high glucose concentration inhib...
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Article
Optimal Production of Poly-γ-glutamic Acid by Metabolically Engineered Escherichia coli
Metabolically-engineered Escherichia coli strains were developed by cloning poly-γ-glutamic acid (γ-PGA) biosynthesis genes, consisting of pgsB, pgsC and pgsA, from Bacillus subtilis The metabolic and regulatory ...
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Article
Characteristics of fed-batch cultures of recombinant Escherichia coli containing human-like collagen cDNA at different specific growth rates
Fed-batch cultures of recombinant Escherichia coli BL21 for producing human-like collagen were performed at different specific growth rates (0.1~0.25 h−1) before induction and at a constant value of 0.05 h−1 afte...
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Article
Poly(3-hydroxybutyrate) synthesis in fed-batch culture of Ralstonia eutropha with phosphate limitation under different glucose concentrations
Poly(3-hydroxybutyrate) (PHB) was produced by fed-batch cultures of Ralstonia eutropha with phosphate limitation under different glucose concentrations. When glucose was kept at 2.5 g l−1, cell growth and PHB syn...