Abstract
High cell density culturing has been conducted for the production of poly(3-hydroxybutyrate) fed-batch cultures ofRalstonia eutropha with phosphate limitation. It was found that a high glucose concentration inhibited the synthesis of P(3HB) in the high cell density culture ofR. eutropha. Although a low glucose concentration can trigger the synthesis of P(3HB) in a manner similar to that of phosphate limitation, it also limited both the P(3HB) synthesis and the cell growth, and led to a low P(3HB) productivity because glucose is the sole carbon source in this reaction. An unstructured model was proposed for predicting the cell growth and P(3HB) synthesis in high cell density cultures ofR. eutropha, where the phosphate concentration played a key role in the accumulation of P(3HB) and in cell growth. Good agreements were found between the experimental data and model predictions. The results of simulation showed that the final P(3HB) concentration would decrease more than 25% when the glucose was concentration increased to 40 g/L, and indicated that the optimal glucose concentration for P(3HB) production by high cell density cultures ofR. eutropha was around 9 g/L.
Similar content being viewed by others
References
Leaf, T. A. and F. Srienc (1998) Metabolic modeling of polyhydroxybutyrate biosynthesis.Biotechnol. Bioeng. 57: 557–570.
Yoo, S. and W. S. Kim (1994) Cybernetic model for synthesis of poly-β-hydroxybutyric acid inAlcaligenes eutrophus.Biotechnol. Bioeng. 43: 1043–1051.
Belfares, L., M. Perrier, B. A. Ramsay, J. A. Ramsay, M. Jolicoeur, and C. Chavarie (1995) Multi-inhibition kinetic model for the growth ofAlcaligenes eutrophus.Can. J. Microbiol. 41: 249–256.
Mulchandani, A., J. H. T. Luong, and C. Groom (1989) Substrate inhibition kinetics for microbial growth and synthesis of poly-β-hydroxybutyric acid byAlcaligenes eutrophus ATCC 17697.Appl. Microbiol. Biotechnol. 30: 11–17.
Patwardhan, P. R. and A. K. Srivastava (2004) Model-based fed-batch cultivation ofR. eutropha for enhanced biopolymer production.Biochem. Eng. J. 20: 21–28.
Shahhosseini, S. (2004) Simulation and optimization of PHB production in fed-batch culture ofRalstonia eutropha.Process Biochem. 39: 963–969.
Tohyama, M., T. Patarinska, Z. Qiang, and K. Shimizu (2002) Modeling of the mixed culture and periodic control for PHB production.Biochem. Eng. J. 10: 157–173.
Katoh, T., D. Yuguchi, H. Yoshii, H. Shi, and K. Shimizu (1999) Dynamics and modeling on fermentative production of poly(β-hydroxybutyric acid) from sugars via lactate by a mixed culture ofLactobacillu delbrueckii andAlcaligenes eutrophus.J. Biotechnol. 67: 113–134.
Ryu, H. W., K. S. Cho, B. S. Kim, Y. K. Chang, H. N. Chang, and H. J. Shim (1999) Mass production of poly(3-hydroxybutyrate) by fed-batch cultures ofRalstonia eutropha with nitrogen and phosphate limitation.J. Microbiol. Biotechnol. 9: 751–756.
Shang, L., M. Jiang, and H. N. Chang (2003) Poly(3-hydroxybutyrate) synthesis in fed-batch culture ofRalstonia eutropha with phosphate limitation under different glucose concentrations.Biotechnol. Lett. 25: 1415–1419.
Dias, J. M., L. S. Serafim, P. C. Lemos, M. A. Reis, and R. Oliveira (2005) Mathematical modelling of a mixed culture cultivation process for the production of polyhydroxybutyrate.Biotechnol. Bioeng. 92: 209–222.
Khanna, S. and A. K. Srivastava (2006) Computer simulated fed-batch cultivation for over production of PHB: A comparison of simultaneous and alternate feeding of carbon and nitrogen.Biochem. Eng. J. 27: 197–203.
Sin, G., A. Guisasola, D. J. W. De Pauw, J. A. Baeza, J. Carrera, and P. A. Vanrolleghem (2005) A new approach for modelling simultaneous storage and growth processes for activated sludge systems under nerobic conditions.Biotechnol. Bioeng. 92: 600–613.
Shang, L., M. Jiang, C. H. Ryu, H. N. Chang, S. H. Cho, and J. W. Lee (2003) Inhibitory effect of carbon dioxide on the fed-batch culture ofRalstonia eutropha: Evaluation by CO2 pulse injection and autogenous CO2 methods.Biotechnol. Bioeng. 83: 312–320.
Lee, J. H., H. C. Lim, and J. Hong (1997) Application of nonsingular transformation to on-line optimal control of poly-β-hydroxybutyrate fermentation.J. Biotechnol. 55: 135–150.
Website of Superprodesigner. http://www.Intelligen. com.
Chu, K. H. and E. Y. Kim (2006) Predictive modeling of competitive biosorption equilibrium data.Biotechnol. Bioprocess Eng. 11: 67–71.
Mahadevan, R., A. P. Burgard, I. Famili, S. V. Dien, and C. H. Schilling (2005) Applications of metabolic modeling to drive bioprocess development for the production of value-added chemicals.Biotechnol. Bioprocess Eng. 10: 408–417.
Lee, Y. M., O. Y. Kwon, and K. G. Ryu (2007) General applications of modified Stokes expression for modeling and scale-up of expanded beds.Kor. J. Chem. Eng. 24: 261–264.
Chen, Y., W. Dai, X. Liu, Y. Cheng, and H. Qu (2006) Kinetic modeling for chromatographic separation of cytosine monophosphate and uracil monophosphate.Kor. J. Chem. Eng. 23: 784–788.
Prakorn, R., P. Weerawat, and P. Ura (2006) Mass transfer modeling of membrane carrier system for extraction of Ce(IV) from sulfate media using hollow fiber supported liquid membrane.Kor. J. Chem. Eng. 23: 85–92.
Aboudzadeh, M. R., Z. Jiawen, and W. Bin (2006) Modeling of protein adsorption to DEAE sepharose FF: Comparison of data with model simulation.Kor. J. Chem. Eng. 23: 124–130.
Wlaschin, A. P., C. T. Trinh, R. Carlson, and F. Srienc (2006) The fractional contributions of elementary modes to the metabolism ofEscherichia coli and their estimation from reaction entropies.Metab. Eng. 8: 338–352.
Jiang, Q. and S. Yao (2007) Predictive modeling of whole-cell bioactivity retention data in the presence of organic compounds.Biotechnol. Bioprocess Eng. 12: 228–234.
Song, B. D., H. Ding, and S. C. Wang (2007) Hydrolysis of olive oil catalyzed by surfactant-coatedCandida rugosa lipase in a hollow fiber membrane reactor.Biotechnol. Bioprocess Eng. 12: 121–124.
Omar, R., M. A. Abdullah, M. A. Hasan, M. Rosfarizan, and M. Marziah (2006) Kinetics and modelling of cell growth and substrate uptake inCentella asiatica cell culture.Biotechnol. Bioprocess Eng. 11: 223–229.
Kwon, Y. J. and C. R. Engler (2005) Kinetic models for growth and product formation on multiple substrates.Biotechnol. Bioprocess Eng. 10: 587–592.
Song, J. Y. and B. S. Kim (2005) Characteristics of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) production byRalstonia eutropha NCIMB 11599 and ATCC 17699.Biotechnol. Bioprocess Eng. 10: 603–606.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Shang, L., Fan, D.D., Kim, M.I. et al. Modeling of poly(3-hydroxybutyrate) production by high cell density fed-batch culture of Ralstonia eutropha . Biotechnol. Bioprocess Eng. 12, 417–423 (2007). https://doi.org/10.1007/BF02931065
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02931065