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Article
Open AccessStructure of orthoreovirus RNA chaperone σNS, a component of viral replication factories
The mammalian orthoreovirus (reovirus) σNS protein is required for formation of replication compartments that support viral genome replication and capsid assembly. Despite its functional importance, a mechanis...
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Chapter and Conference Paper
Attention-Based RNA Secondary Structure Prediction
RNA is a molecule composed of ribonucleotides and plays a crucial role in biological activities. The computational prediction of RNA secondary structures has been a long-standing issue in computational biology...
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Article
Open AccessA single nanobody neutralizes multiple epochally evolving human noroviruses by modulating capsid plasticity
Acute gastroenteritis caused by human noroviruses (HuNoVs) is a significant global health and economic burden and is without licensed vaccines or antiviral drugs. The GII.4 HuNoV causes most epidemics worldwid...
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Article
Open AccessAnti-signal recognition particle positive necrotizing myopathy-sjogren’s syndrome overlap syndrome: a descriptive study on clinical and myopathology features
The aim of this study was to elucidate the clinical and myopathological characteristics of patients with anti-signal recognition particle (SRP) positive immune-mediated necrotizing myopathy (IMNM) overlap Sjog...
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Article
Open AccessMap** the determinants of catalysis and substrate specificity of the antibiotic resistance enzyme CTX-M β-lactamase
CTX-M β-lactamases are prevalent antibiotic resistance enzymes and are notable for their ability to rapidly hydrolyze the extended-spectrum cephalosporin, cefotaxime. We hypothesized that the active site seque...
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Article
Open AccessAn active site loop toggles between conformations to control antibiotic hydrolysis and inhibition potency for CTX-M β-lactamase drug-resistance enzymes
β-lactamases inactivate β-lactam antibiotics leading to drug resistance. Consequently, inhibitors of β-lactamases can combat this resistance, and the β-lactamase inhibitory protein (BLIP) is a naturally occurr...
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Article
Open AccessNovel fold of rotavirus glycan-binding domain predicted by AlphaFold2 and determined by X-ray crystallography
The VP8* domain of spike protein VP4 in group A and C rotaviruses, which cause epidemic gastroenteritis in children, exhibits a conserved galectin-like fold for recognizing glycans during cell entry. In group ...
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Article
Open AccessNANOG prion-like assembly mediates DNA bridging to facilitate chromatin reorganization and activation of pluripotency
Human NANOG expression resets stem cells to ground-state pluripotency. Here we identify the unique features of human NANOG that relate to its dose-sensitive function as a master transcription factor. NANOG is ...
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Article
Open AccessAtomic structure of the predominant GII.4 human norovirus capsid reveals novel stability and plasticity
Human noroviruses (HuNoVs) cause sporadic and epidemic viral gastroenteritis worldwide. The GII.4 variants are responsible for most HuNoV infections, and GII.4 virus-like particles (VLPs) are being used in vac...
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Article
Open AccessAuthor Correction: Broadly cross-reactive human antibodies that inhibit genogroup I and II noroviruses
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Article
Open AccessBroadly cross-reactive human antibodies that inhibit genogroup I and II noroviruses
The rational development of norovirus vaccine candidates requires a deep understanding of the antigenic diversity and mechanisms of neutralization of the virus. Here, we isolate and characterize a panel of bro...
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Article
Open AccessStructural basis of the stereoselective formation of the spirooxindole ring in the biosynthesis of citrinadins
Prenylated indole alkaloids featuring spirooxindole rings possess a 3R or 3S carbon stereocenter, which determines the bioactivities of these compounds. Despite the stereoselective advantages of spirooxindole bio...
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Chapter and Conference Paper
Cryo-EM Structure of Rotavirus VP3 Reveals Novel Insights into Its Role in RNA Cap** and Endogenous Transcription
Rotavirus, a global causative agent of life-threatening gastroenteritis, is a double-stranded RNA (dsRNA) virus in the family Reoviridae. A common feature in this family of viruses, containing multiple dsRNA s...
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Article
Open AccessPodoplanin is a useful prognostic marker and indicates better differentiation in lung squamous cell cancer patients? A systematic review and meta-analysis
The CSC (cancer stem cell) markers often indicate poor prognosis and more cell invasion or migration of cancer patients. Podoplanin was assumed as a candidate CSC marker and predict poor prognosis among squamo...
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Article
Structure and mechanism of human diacylglycerol O-acyltransferase 1
Diacylglycerol O-acyltransferase 1 (DGAT1) synthesizes triacylglycerides and is required for dietary fat absorption and fat storage in humans1. DGAT1 belongs to the membrane-bound O-acyltransferase (MBOAT) superf...
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Article
Open AccessTrkA undergoes a tetramer-to-dimer conversion to open TrkH which enables changes in membrane potential
TrkH is a bacterial ion channel implicated in K+ uptake and pH regulation. TrkH assembles with its regulatory protein, TrkA, which closes the channel when bound to ADP and opens it when bound to ATP. However, it ...
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Article
Open AccessHuman milk oligosaccharides, milk microbiome and infant gut microbiome modulate neonatal rotavirus infection
Neonatal rotavirus infections are predominantly asymptomatic. While an association with gastrointestinal symptoms has been described in some settings, factors influencing differences in clinical presentation a...
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Article
Open AccessDifferential active site requirements for NDM-1 β-lactamase hydrolysis of carbapenem versus penicillin and cephalosporin antibiotics
New Delhi metallo-β-lactamase-1 exhibits a broad substrate profile for hydrolysis of the penicillin, cephalosporin and ‘last resort’ carbapenems, and thus confers bacterial resistance to nearly all β-lactam an...
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Article
The impact of UGT2B7 C802T and CYP3A4*1G polymorphisms on pain relief in cancer patients receiving oxycontin
Single nucleotide polymorphisms (SNPs) in UGT2B7 C802T and CYP3A4*1G impact drug metabolism. This study aimed to investigate the impact of these SNPs on the efficacy of oxycontin for pain relief in cancer patient...
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Article
Open AccessGlycan recognition in globally dominant human rotaviruses
Rotaviruses (RVs) cause life-threatening diarrhea in infants and children worldwide. Recent biochemical and epidemiological studies underscore the importance of histo-blood group antigens (HBGA) as both cell a...
