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Article
Genetic propensities to increase ethanol intake in response to stress: studies with selectively bred swim test susceptible (SUS), alcohol-preferring (P), and non-preferring (NP) lines of rats
Swim test susceptible (SUS) rats selectively bred for reduced struggling in the forced swim test (FST) following stress show high voluntary ethanol intake like alcohol-preferring (P) rats selectively bred for ...
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Article
Effects of sazetidine-A, a selective α4β2 nicotinic acetylcholine receptor desensitizing agent on alcohol and nicotine self-administration in selectively bred alcohol-preferring (P) rats
Manipulations of nicotinic cholinergic receptors have been shown to influence both alcohol and nicotine intake. Sazetidine-A [6-(5(((S)-azetidine-2-yl)methoxy)pyridine-3-yl)hex-5-yn-1-ol] is a novel compound that...
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Article
Development of Congenic Rat Strains for Alcohol Consumption Derived from the Alcohol-Preferring and Nonpreferring Rats
A genome scan of the F2 generation from an inbred alcohol-preferring (iP) and inbred alcohol-nonpreferring (iNP) rat cross identified a significant quantitative trait locus (QTL) on chromosome 4 with a lod sco...
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Article
Identification of QTLs Influencing Alcohol Preference in the High Alcohol Preferring (HAP) and Low Alcohol Preferring (LAP) Mouse Lines
The High- and Low-Alcohol Preferring (HAP1/LAP1 and HAP2/LAP2) mouse lines were developed by selective breeding for differences in alcohol preference. They represent the only extant selectively bred mouse line...
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Article
Effects of Repeated Alcohol Deprivations on Operant Ethanol Self-Administration by Alcohol-Preferring (P) Rats
We reported that repeated alcohol deprivations prolonged the expression of an alcohol-deprivation effect (ADE) under 24-h free-choice alcohol-drinking access and that the duration of the initial deprivation pe...
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Article
Phenotypic and Genotypic Characterization of the Indiana University Rat Lines Selectively Bred for High and Low Alcohol Preference
The Indiana lines of selected rats, the HAD and LAD replicates and the P and NP lines, were bred for high and low alcohol preference. The P and HAD lines have met criteria for an animal model of alcoholism in ...
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Article
Identification of Quantitative Trait Loci Influencing Alcohol Consumption in the High Alcohol Drinking and Low Alcohol Drinking Rat Lines
Selective breeding has been employed to develop high-alcohol-drinking (HAD) and low-alcohol-drinking (LAD) rat lines from the heterogeneous N/Nih rat. Within-family selection and a rotational breeding design w...
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Article
Selective Breeding for High and Low Alcohol Preference in Mice
High and low alcohol preference (HAP and LAP, respectively) mice were created by 10 generations of bidirectional selection for differences in two-bottle choice alcohol consumption. The progenitors used for sel...
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Article
Genomic screen for QTLs underlying alcohol consumption in the P and NP rat lines
Selective breeding for voluntary alcohol consumption was utilized to establish the alcohol-preferring (P) and alcohol-nonpreferring (NP) rat lines. Inbreeding was initiated after 30 generations of selection a...
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Article
Quantitative autoradiography on [35S]TBPS binding sites of gamma-aminobutyric acidA receptors in discrete brain regions of high-alcohol-drinking and low-alcohol-drinking rats selectively bred for high- and low-alcohol preference
It has been documented that ethanol can potentiate brain γ-aminobutyric acid (GABA)ergic function, and there is a close link between the GABAA receptor complex and effects of ethanol, including reinforcement of a...
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Article
The benzodiazepine inverse agonist RO19-4603 exerts prolonged and selective suppression of ethanol intake in alcohol-preferring (P) rats
The time course of the benzodiazepine (BDZ) inverse agonist RO19-4603 in antagonizing ethanol (EtOH) intake was investigated in alcohol-preferring (P) rats (n=7) maintained on 24-h continuous free-choice access t...
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Article
Viral hepatitis (HBV and HCV) background in Chinese patients with hepatocellular carcinoma
Antibody to hepatitis C virus (Anti-HCV) was detected using anti-HCV EIA (Abbott Kit) in the sera of Chinese patients with hepatocellular carcinoma (HCC), acute hepatitis, chronic hepatitis and blood donors, t...
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Article
Selective breeding for alcohol preference and associated responses
Selective breedng for high and low alcohol-drinking preference has produced rat lines useful for studying the nature of excessive alcohol-seeking behavior. Compared with the alcohol-nonpreferring line, the pre...
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Chapter
Protein-Acetaldehyde Adducts as Biochemical Markers of Alcohol Consumption
There are two types of diagnostic markers for alcohol abuse and alcoholism: trait (or vulnerability) markers and state markers.1,2 Trait markers include indicators that will identify individuals who are more vuln...
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Chapter
Formation of a Protein-Acetaldehyde Adduct in Liver during Chronic Alcohol Exposure
Acetaldehyde is an intermediary metabolite of ethanol oxidation that forms in the liver during alcohol ingestion. Formation of acetaldehyde is mediated by two alcohol metabolizing systems: alcohol dehydrogenas...
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Chapter
Molecular and Genetic Approaches to Understanding Alcohol-Seeking Behavior
In alcoholism research, two fundamental and related questions are: “why do people drink?” and“why do some people drink too much despite having experienced negative consequences?” Drinking normally occurs in a ...
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Chapter
Alcohol and Hepatic Protein Modification
Although a small percentage of alcohol, after ingestion, can be excreted unchanged in breath and urine, most of its elimination is carried out by enzymatic oxidation. Studies with experimental animals have sho...
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Chapter
Mechanism(s) of Modulation of Alcohol Consumption: Studies on the P and NP Rats
In several laboratories, lines of rats have been selectively bred for disparate alcohol drinking preferences. Historically, the first lines of rats selectively bred were the UChA (low-alcohol consumer) and UCh...
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Chapter
Serotonin and Ethanol Preference
This chapter brings together evidence indicating the involvement of serotonin (5-HT) in ethanol preference using data mainly obtained from selectively bred alcohol-preferring and alcohol-nonpreferring lines of...
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Chapter
Microassay of Pyridoxal Phosphate Using Tyrosine Apodecarboxylase
Pyridoxal 5′-phosphate (PLP) assay based on the coenzyme-dependent decarboxylation of L-tyrosine by tyrosine apodecarboxylase (EC 4.1.1.25) was the first enzymatic method developed in vitamin B-6 analysis. Ori...
