16 Result(s)
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Lacey E. Dobrolecki
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Article
Open AccessImmunologically “cold” triple negative breast cancers engraft at a higher rate in patient derived xenografts
TNBC is a heterogeneous subtype of breast cancer, and only a subset of TNBC can be established as PDXs. Here, we show that there is an engraftment bias toward TNBC with low levels of immune cell infiltration. ...
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Author Correction: PHGDH heterogeneity potentiates cancer cell dissemination and metastasis
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Open AccessCorrection to: In Vivo Modeling of Human Breast Cancer Using Cell Line and Patient-Derived Xenografts
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Open AccessIn Vivo Modeling of Human Breast Cancer Using Cell Line and Patient-Derived Xenografts
Historically, human breast cancer has been modeled largely in vitro using long-established cell lines primarily in two-dimensional culture, but also in three-dimensional cultures of varying cellular and molecu...
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PHGDH heterogeneity potentiates cancer cell dissemination and metastasis
Cancer metastasis requires the transient activation of cellular programs enabling dissemination and seeding in distant organs1. Genetic, transcriptional and translational heterogeneity contributes to this dynamic...
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Open AccessA human breast cancer-derived xenograft and organoid platform for drug discovery and precision oncology
Models that recapitulate the complexity of human tumors are urgently needed to develop more effective cancer therapies. We report a bank of human patient-derived xenografts (PDXs) and matched organoid cultures...
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Protocol
Surgical Procedure for Implantation of Human Tumor Tissue into the Epithelium-Free Mammary Fat Pad of Immunocompromised Mice to Generate Patient-Derived Xenografts (PDX)
Implantation of cancerous tissue obtained from patient biopsies or surgical procedures to create patient-derived xenograft models (PDX) has greatly enhanced the ability of scientists and physicians to perform ...
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Article
C/EBPδ links IL-6 and HIF-1 signaling to promote breast cancer stem cell-associated phenotypes
To improve cancer patient outcome significantly, we must understand the mechanisms regulating stem-like cancer cells, which have been implicated as a cause of metastasis and treatment resistance. The transcrip...
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Open AccessCirculating tumor cell investigation in breast cancer patient-derived xenograft models by automated immunofluorescence staining, image acquisition, and single cell retrieval and analysis
Breast cancer patient-derived xenograft (BC-PDX) models represent a continuous and reproducible source of circulating tumor cells (CTCs) for studying their role in tumor biology and metastasis. We have previou...
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Open AccessDifferentiation-state plasticity is a targetable resistance mechanism in basal-like breast cancer
Intratumoral heterogeneity in cancers arises from genomic instability and epigenomic plasticity and is associated with resistance to cytotoxic and targeted therapies. We show here that cell-state heterogeneity...
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Article
Combinatorial inhibition of PTPN12-regulated receptors leads to a broadly effective therapeutic strategy in triple-negative breast cancer
Targeting tyrosine kinase receptors that share the feedback inhibitor PTPN12 leads to broad spectrum therapeutic suppression of triple-negative breast cancer.
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Mutual regulation of tumour vessel normalization and immunostimulatory reprogramming
The cross-talk between immune cells and blood vessel endothelial cells promotes pericyte coverage and decreases hypoxia in mouse tumour models, and correlative evidence suggests that these processes influence ...
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Patient-derived xenograft (PDX) models in basic and translational breast cancer research
Patient-derived xenograft (PDX) models of a growing spectrum of cancers are rapidly supplanting long-established traditional cell lines as preferred models for conducting basic and translational preclinical re...
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Erratum: Oncogenic mTOR signalling recruits myeloid-derived suppressor cells to promote tumour initiation
Nature Cell Biology 18, 632–644 (2016); published online 16 May 2016; corrected after print 20 May 2016 In the version of this Article originally published, in the fourth affiliation, 'Los Angeles' should have...
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Article
Oncogenic mTOR signalling recruits myeloid-derived suppressor cells to promote tumour initiation
Myeloid-derived suppressor cells (MDSCs) play critical roles in primary and metastatic cancer progression. MDSC regulation is widely variable even among patients harbouring the same type of malignancy, and the...
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Open AccessCirculating and disseminated tumor cells from breast cancer patient-derived xenograft-bearing mice as a novel model to study metastasis
Real-time monitoring of biologic changes in tumors may be possible by investigating the transitional cells such as circulating tumor cells (CTCs) and disseminated tumor cells in bone marrow (BM-DTCs). However,...
