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Article
Open AccessFrontline perspectives on barriers to care for patients with California Medicaid: a qualitative study
While insurance is integral for accessing healthcare in the US, coverage alone may not ensure access, especially for those publicly insured. Access barriers for Medicaid-insured patients are rooted in social d...
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Article
Engineering a synthesis-friendly serum responsive promoter for antibiotic selection of genomic integration in cell-based therapy
For clinical cell-based therapies (e.g. CAR-T cells), the genomic integration of therapeutic genes into cells are selected using inefficient resistance genes of host origin to avoid potential immune response f...
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Article
Generation of stable cell lines using readthrough expression from lentiviral integration
Lentiviral infection is often used to integrate genetic material into cells to stably express transgenes of interest. Depending on the location of integration into the host genome, readthrough expression of th...
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Chapter
Advances in Senotherapies
Aging brings about many risks factors that can lead to age-related chronic disorders such as atherosclerosis, osteoporosis, and neurodegenerative diseases. Implicated in aging and age-related pathologies, the ...
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Protocol
LOV2-Controlled Photoactivation of Protein Trans-Splicing
Protein trans-splicing is a posttranslational modification that joins two protein fragments together via a peptide a bond in a process that does not require exogenous cofactors. Towards achieving cellular control...
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Article
Open AccessModular assembly of synthetic proteins that span the plasma membrane in mammalian cells
To achieve synthetic control over how a cell responds to other cells or the extracellular environment, it is important to reliably engineer proteins that can traffic and span the plasma membrane. Using a modul...
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Article
Genomic integration occurs in the packaging cell via unexported lentiviral precursors
To use HIV-1 based lentivirus components to produce gene integration and the formation of a stable cell line in the packaging cell line without viral infection.
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Chapter and Conference Paper
Development of Reduced Order Models to Non-modeled Regions
Model reduction and model expansion techniques have been used in many structural dynamic modeling applications to map detailed FEM at the full set of DOF (NDOF) to an abbreviated set of active DOF (ADOF) while...
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Protocol
Design of Fluorescent Fusion Protein Probes
Many fluorescent probes depend on the fluorescence resonance energy transfer (FRET) between fluorescent protein pairs. The efficiency of energy transfer becomes altered by conformational changes of a fused sen...
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Article
Open AccessCascading signaling pathways improve the fidelity of a stochastically and deterministically simulated molecular RS latch
While biological systems have often been compared with digital systems, they differ by the strong effect of crosstalk between signals due to diffusivity in the medium, reaction kinetics and geometry. Memory el...
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Article
Open Access160-fold acceleration of the Smith-Waterman algorithm using a field programmable gate array (FPGA)
To infer homology and subsequently gene function, the Smith-Waterman (SW) algorithm is used to find the optimal local alignment between two sequences. When searching sequence databases that may contain hundred...
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Protocol
Calcium Indicators Based on Calmodulin-Fluorescent Protein Fusions
Calmodulin (CaM) is an ubiquitous protein involved in Ca2+-mediated signal transduction. On Ca2+ influx, CaM acquires a strong affinity to various cellular proteins with one or more CaM recognition sequences, res...
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Chapter
Current Approaches for Engineering Proteins with Diverse Biological Properties
In the past two decades, protein engineering has advanced significantly with the emergence of new chemical and, genetic approaches. Modification and recombination of existing proteins not only produced novel e...
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Article
Protein biosensors based on the principle of fluorescence resonance energy transfer for monitoring cellular dynamics
Genetically-coded, fluorescence resonance energy transfer (FRET) biosensors are widely used to study molecular events from single cells to whole organisms. They are unique among biosensors because of their spo...
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Article
Creation of Circularly Permutated Yellow Fluorescent Proteins Using Fluorescence Screening and a Tandem Fusion Template
By experimenting with many different circularly permutated yellow fluorescent protein (cpYFP) variants as acceptors in fluorescence resonance energy transfer based biosensors, the optimal dynamic range can be ...
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Article
Using Co-Cultures Expressing Fluorescence Resonance Energy Transfer Based Protein Biosensors to Simultaneously Image Caspase-3 and Ca2+ Signaling
Fluorescence resonance energy transfer (FRET)-based protein biosensors allow the spatial and temporal imaging of signaling events in living cells. However, the simultaneous correlation of multiple events of a ...
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Article
Open AccessA fluorescent cassette-based strategy for engineering multiple domain fusion proteins
The engineering of fusion proteins has become increasingly important and most recently has formed the basis of many biosensors, protein purification systems, and classes of new drugs. Currently, most fusion pr...
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Article
Open AccessDomain fusion analysis by applying relational algebra to protein sequence and domain databases
Domain fusion analysis is a useful method to predict functionally linked proteins that may be involved in direct protein-protein interactions or in the same metabolic or signaling pathway. As separate domain d...
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Article
The cadherin superfamily database
The cadherin superfamily is a large protein family with diverse structures and functions. Because of this diversity and the growing biological interest in cell adhesion and signaling processes, in which many m...
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Article
Identification and characterization of subfamily-specific signatures in a large protein superfamily by a hidden Markov model approach
Most profile and motif databases strive to classify protein sequences into a broad spectrum of protein families. The next step of such database studies should include the development of classification systems ...
