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Article
Open AccessCep164, but not EB1, is critical for centriolar localization of TTBK2 and its function in ciliogenesis
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Protocol
A Novel Pair of Split Venus Fragments to Detect Protein–Protein Interactions by In Vitro and In Vivo Bimolecular Fluorescence Complementation Assays
Protein–protein interactions are critical components of almost every cellular process. The bimolecular fluorescence complementation (BiFC) method has been used to detect protein–protein interactions in both li...
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Article
F- and G-actin homeostasis regulates mechanosensitive actin nucleation by formins
Physical force evokes rearrangement of the actin cytoskeleton. Signalling pathways such as tyrosine kinases, stretch-activated Ca2+ channels and Rho GTPases are involved in force sensing. However, how signals are...
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Article
Protein kinase D1 regulates cofilin-mediated F-actin reorganization and cell motility through slingshot
The actin severing factor cofilin is activated by the slingshot phosphatases. Phosphorylation of slingshot 1L by protein kinase D is found to block the association of slingshot 1L with actin, thereby inhibitin...
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Article
Phosphorylation of cofilin by LIM-kinase is necessary for semaphorin 3A-induced growth cone collapse
Semaphorin 3A is a chemorepulsive axonal guidance molecule that depolymerizes the actin cytoskeleton and collapses growth cones of dorsal root ganglia neurons. Here we investigate the role of LIM-kinase 1, whi...
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Article
Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization
Rac is a small GTPase of the Rho family that mediates stimulus-induced actin cytoskeletal reorganization to generate lamellipodia1,2,3,4,5. Little is known about the signalling pathways that link Rac activation t...
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Article
Inhibition of activated Ras-induced neuronal differentiation of PC12 cells by the LIM domain of LIM-kinase 1
LIM-kinase 1 and 2 (LIMK1 and LIMK2) are members of a novel class of protein kinases with structures composed of two LIM motifs at the N-terminus and an unusual protein kinase domain at the C-terminus. The cel...
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Article
A novel protease from yeast with specificity towards paired basic residues
Paired basic residues have been observed as sites of proteolytic processing of prohormones in a wide range of eukaryotic species1–3. This strongly suggests that proteases exhibiting specificity towards paired bas...
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Article
Novel C-terminally amidated opioid peptide in human phaeochromocytoma tumour
As has often been observed in hypothalamic releasing factors and gastrointestinal hormones, the carboxy-terminal amide structure is a unique feature of peptides exhibiting hormonal or physiological activities....