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  1. No Access

    Protocol

    Affinity Purification of Intraflagellar Transport (IFT) Proteins in Mice Using Endogenous Streptavidin/FLAG Tags

    Biological complexity is achieved through elaborate interactions between relatively few individual components. Affinity purification (AP) has allowed these networks of protein-protein interactions that regulat...

    Tina Beyer, Tiago Martins, Jeshmi Jeyabalan Srikaran, Marian Seda, Emma Peskett… in Cilia (2024)

  2. Article

    Open Access

    The Na+-activated K+ channel Slack contributes to synaptic development and plasticity

    Human mutations of the Na+-activated K+ channel Slack (KCNT1) are associated with epilepsy and intellectual disability. Accordingly, Slack knockout mice (Slack−/−) exhibit cognitive flexibility deficits in distin...

    Lucas Matt, Thomas Pham, David Skrabak… in Cellular and Molecular Life Sciences (2021)

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  3. Article

    Open Access

    Characterizing the morbid genome of ciliopathies

    Ciliopathies are clinically diverse disorders of the primary cilium. Remarkable progress has been made in understanding the molecular basis of these genetically heterogeneous conditions; however, our knowledge...

    Ranad Shaheen, Katarzyna Szymanska, Basudha Basu, Nisha Patel, Nour Ewida… in Genome Biology (2016)

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  4. Article

    Open Access

    KIAA0556 is a novel ciliary basal body component mutated in Joubert syndrome

    Joubert syndrome (JBTS) and related disorders are defined by cerebellar malformation (molar tooth sign), together with neurological symptoms of variable expressivity. The ciliary basis of Joubert syndrome rela...

    Anna A. W. M. Sanders, Erik de Vrieze, Anas M. Alazami, Fatema Alzahrani… in Genome Biology (2015)

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  5. No Access

    Article

    An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes

    Defects in primary cilium biogenesis underlie the ciliopathies, a growing group of genetic disorders. We describe a whole-genome siRNA-based reverse genetics screen for defects in biogenesis and/or maintenance...

    Gabrielle Wheway, Miriam Schmidts, Dorus A. Mans… in Nature Cell Biology (2015)

  6. Article

    Open Access

    Expression of leukemia inhibitory factor in Müller glia cells is regulated by a redox-dependent mRNA stability mechanism

    Photoreceptor degeneration is a main hallmark of many blinding diseases making protection of photoreceptors crucial to prevent vision loss. Thus, regulation of endogenous neuroprotective factors may be key for...

    Cavit Agca, Karsten Boldt, Andrea Gubler, Isabelle Meneau, Armelle Corpet… in BMC Biology (2015)

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  7. No Access

    Protocol

    Applying SILAC for the Differential Analysis of Protein Complexes

    Pull-downs based on tag fusion proteins as well as immunoprecipitations (IP) are widely used methods to analyze protein interactions. Selectivity and specificity of both methods are compromised by nonspecific ...

    Karsten Boldt, Christian J. Gloeckner… in Stable Isotope Labeling by Amino Acids in … (2014)

  8. No Access

    Protocol

    Tandem Affinity Purification of Protein Complexes from Mammalian Cells by the Strep/FLAG (SF)-TAP Tag

    Isolation and dissection of native multiprotein complexes is a central theme in functional genomics. The development of the tandem affinity purification (TAP) tag has enabled efficient and large-scale purificatio...

    Christian Johannes Gloeckner, Karsten Boldt, Annette Schumacher… in Proteomics (2009)