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Article
Open AccessHigh frequency in vitro regeneration of adventitious shoots in daylilies (Hemerocallis sp) stem tissue using thidiazuron
Daylilies are a lucrative crop used for its floral beauty, medicinal proprieties, landsca**, fire prevention, nutritional value, and research. Despite the importance, daylilies remain extremely challenging f...
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Article
Decreased Na+/K+ ATPase α1 (ATP1A1) gene expression in major depression patients’ peripheral blood
Major depression affects the central nervous system and thereafter the autonomic nervous system, immune system, and endocrine system. Na+/K+ ATPase, as a major mediator of cellular transmembrane ionic gradient...
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Article
De novo next-generation sequencing, assembling and annotation of Arachis hypogaea L. Spanish botanical type whole plant transcriptome
Peanut is a major agronomic crop within the legume family and an important source of plant oil, proteins, vitamins, and minerals for human consumption, as well as animal feed, bioenergy, and health products. P...
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Article
Natural rules for Arabidopsis thaliana pre-mRNA splicing site selection
The accurate prediction of plant pre-mRNA splicing sites has been studied extensively. The rules for plant pre-mRNA splicing still remain unknown. This study, based on confirmed sequence data, systematically a...
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Article
Open AccessA Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf
Subtraction technique has been broadly applied for target gene discovery. However, most current protocols apply relative differential subtraction and result in great amount clone mixtures of unique and differe...
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Article
Cloning, Sequence and Functional Analysis of Goat ATP-binding Cassette Transporter G2 (ABCG2)
ATP-binding cassette transporter G2 (ABCG2) gene encodes a protein that has a wide variety of substrates and is responsible for the active secretion of clinically and toxicologically important molecules into m...
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Article
Construction and Characterization of a Goat Mammary Gland cDNA Library
A lactating goat mammary gland cDNA library was constructed by using a modified commercially available cDNA library construction kit protocol. The resulting clones were sequenced and functionally analyzed thro...
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Article
Normal/disease-paired cDNA library subtraction for molecular marker development
This study consists of a novel strategy for the identification of potential cancer exclusively expressed genes, which might lead to the development of valuable diagnostic molecular markers. Normal- and cancerp...