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Article
Memristor device based on bioengineered elastin-like polypeptide and its bionanohybrid
In this study, bioengineered and biosynthesized elastin-like polypeptide (ELP) was adopted for a non-volatile memory resistive switching device or a memristor. The ELP mimicked from elastin of mammal was synth...
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Article
Thermoresponsive, dually cross-linked elastin-like-polypeptide (ELP) micelle hydrogel with recovery properties
Thermoresponsive protein-based hydrogels have been widely used due to their high potential in biomedical fields. Elastin-like polypeptides (ELPs) are one of the proteins that show lower critical solution tempe...
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Article
Development of a Pumpless Microfluidic System to Study the Interaction between Gut Microbes and Intestinal Epithelial Cells
Gut microbiota dysbiosis and intestinal epithelial cell barrier dysfunction are associated with diseases such as inflammatory bowel disease and type 2 diabetes. To study the interaction of gut microbes and int...
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Article
Characterization of Thermo-sensitive ELP-liposome Complexes According to the Conjugation Manner
Elastin-like polypeptides (ELPs) are thermoresponsive protein-based biopolymers that exhibit phase transition behavior. By conjugating them to the liposome surface, temperature-sensitive ELP-liposome complexes...
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Article
Characterization of Amphiphilic Elastin-like Polypeptide (ELP) Block Copolymers as Drug Delivery Carriers
Elastin-like polypeptides (ELPs) are stimulusresponsive protein-based biopolymers that exhibit phase transition behavior. Some ELP block copolymers can assemble spherical nanoparticles with thermo-sensitivity....
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Article
Characterization of ELP-fused ω-Transaminase and Its Application for the Biosynthesis of β-Amino Acid
Optically pure amines, β-amino acids and γ-amino acids are the valuable precursors to produce biologically active compounds. The ω-TAs are the class of enzymes which are widely used to produce such compounds. ...
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Article
Synthesis and characterization of an ELP-conjugated liposome with thermo-sensitivity for controlled release of a drug
Liposome, one of various drug carriers, has been extensively studied as an inert carrier for the delivery of protein, DNA, and biologically active agents into cells. Recently, much effort has been directed to ...
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Article
Engineering thermal properties of elastin-like polypeptides by incorporation of unnatural amino acids in a cell-free protein synthesis system
Based on the central dogma of protein synthesis, traditional methods for protein engineering require that altering protein structure and function must be accompanied by changing the nucleotide sequence of the ...
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Article
Influence of Vitreoscilla hemoglobin gene expression on 2,3-butanediol production in Klebsiella oxytoca
A hemoglobin-like protein is a protein that aids in the delivery of oxygen in microbes in a similar fashion as hemoglobins in plants and animals. In this study, expression of Vitreoscilla hemoglobin (VHb) was use...
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Article
Validation of 4 type-STR analysis for identification of 50 Korean
Short tandem repeat (STR) analysis provides genetic fingerprinting of individuals and is an indispensable technique for forensic human identification. Recently, this technique has been used in social areas, su...
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Article
A new cloning strategy for generating multiple repeats of a repetitive polypeptide based on non-template PCR
A new cloning method for generating multiple repeats of amino acids is described which can be used as biomaterials, protein polymers and biomedical applications. Although several traditional methods for clonin...
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Article
Increased ethanol resistance in Ethanolic Escherichia coli by Insertion of heat-shock genes BEM1 and SOD2 from Saccharomyces cerevisiae
Ethanol is generally toxic to microorganisms, and intracellular and extracellular accumulation of ethanol inhibits cell growth and metabolism. In this study, pyruvate decarboxylase (pdc) and alcohol dehydrogen...
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Article
Thermal behaviors of elastin-like polypeptides (ELPs) according to their physical properties and environmental conditions
Elastin-like polypeptides (ELPs) have a distinctive thermal property, transition temperature (Tt), which leads to phase transition. This thermal property depends on the molecular weight (MW) of ELP, ELP concentra...
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Article
Analysis and comparison of cloning methods for the preparation of repetitive polypeptides
Repetitive polypeptides, defined as a protein consisting primarily of tandemly repeated blocks of amino acid sequence, are widely used biomaterials. These repetitive polypeptides can be used in diverse biologi...
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Article
Recent advances in DNA sequencing by End-Labeled Free-Solution Electrophoresis (ELFSE)
End-Labeled Free-Solution Electrophoresis (ELFSE) is a new technique that is a promising bioconjugate method for DNA sequencing (or separation) and genoty** by both capillary and microfluidic device electrop...