Page
%P
-
Article
Open AccessGlucose-methanol co-utilization in Pichia pastoris studied by metabolomics and instationary 13C flux analysis
Several studies have shown that the utilization of mixed carbon feeds instead of methanol as sole carbon source is beneficial for protein production with the methylotrophic yeast Pichia pastoris. In particular, g...
-
Article
Open AccessMetabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures
The methylotrophic yeast Pichia pastoris has emerged as one of the most promising yeast hosts for the production of heterologous proteins. Mixed feeds of methanol and a multicarbon source instead of methanol as s...
