-
Article
Open AccessA CRISPR activation and interference toolkit for industrial Saccharomyces cerevisiae strain KE6-12
Recent advances in CRISPR/Cas9 based genome editing have considerably advanced genetic engineering of industrial yeast strains. In this study, we report the construction and characterization of a toolkit for C...
-
Article
Open AccessIncreased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris
The methanol-regulated alcohol oxidase promoter (PAOX1) of Pichia pastoris is one of the strongest promoters for heterologous gene expression in this methylotrophic yeast. Although increasing gene dosage is one o...
-
Article
Open AccessMetabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures
The methylotrophic yeast Pichia pastoris has emerged as one of the most promising yeast hosts for the production of heterologous proteins. Mixed feeds of methanol and a multicarbon source instead of methanol as s...
