10 Result(s)
within
Jiahai Zhou
Science, Humanities and Social Sciences, multidisciplinary
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Article
Open AccessReductive inactivation of the hemiaminal pharmacophore for resistance against tetrahydroisoquinoline antibiotics
Antibiotic resistance is becoming one of the major crises, among which hydrolysis reaction is widely employed by bacteria to destroy the reactive pharmacophore. Correspondingly, antibiotic producer has canonic...
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Article
An enzymatic Alder-ene reaction
An ongoing challenge in chemical research is to design catalysts that select the outcomes of the reactions of complex molecules. Chemists rely on organocatalysts or transition metal catalysts to control stereo...
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Article
Open AccessArtificial cysteine-lipases with high activity and altered catalytic mechanism created by laboratory evolution
Engineering artificial enzymes with high activity and catalytic mechanism different from naturally occurring enzymes is a challenge in protein design. For example, many attempts have been made to obtain active...
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Article
Resistance-gene-directed discovery of a natural-product herbicide with a new mode of action
Bioactive natural products have evolved to inhibit specific cellular targets and have served as lead molecules for health and agricultural applications for the past century1–3. The post-genomics era has brought a...
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Article
Open AccessGyrI-like proteins catalyze cyclopropanoid hydrolysis to confer cellular protection
GyrI-like proteins are widely distributed in prokaryotes and eukaryotes, and recognized as small-molecule binding proteins. Here, we identify a subfamily of these proteins as cyclopropanoid cyclopropyl hydrola...
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Article
Open AccessCrystal structure of tyrosine decarboxylase and identification of key residues involved in conformational swing and substrate binding
Tyrosine decarboxylase (TDC) is a pyridoxal 5-phosphate (PLP)-dependent enzyme and is mainly responsible for the synthesis of tyramine, an important biogenic amine. In this study, the crystal structures of the...
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Article
Open AccessThe structural basis of an NADP+-independent dithiol oxidase in FK228 biosynthesis
The disulfide bond is unusual in natural products and critical for thermal stability, cell permeability and bioactivity. DepH from Chromobacterium violaceum No. 968 is an FAD-dependent enzyme responsible for cata...
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Article
Open AccessThe mutation network for the hemagglutinin gene from the novel influenza A (H1N1) virus
A mutation network for the hemagglutinin gene (HA) of the novel type A (H1N1) influenza virus was constructed. Sequence homology analysis indicated that one HA sequence type from the viruses mainly isolated from ...
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Article
Open AccessSubty** of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR
The outbreak of a novel influenza A (H1N1) virus across the globe poses a threat to human health. It is of paramount importance to develop a rapid, reliable and inexpensive diagnostic procedure. Based on the b...
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Article
Open AccessStructure modeling and spatial epitope analysis for HA protein of the novel H1N1 influenza virus
In recent months, a novel influenza virus H1N1 broke out around the world. With bioinformatics technology, the 3D structure of HA protein was obtained, and the epitope residues were predicted with the method d...
