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Article
Open AccessChromobacterium spp. mediate their anti-Plasmodium activity through secretion of the histone deacetylase inhibitor romidepsin
The Chromobacterium sp. Panama bacterium has in vivo and in vitro anti-Plasmodium properties. To assess the nature of the Chromobacterium-produced anti-Plasmodium factors, chemical partition was conducted by bioa...
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Article
Open AccessTarget engagement and drug residence time can be observed in living cells with BRET
The therapeutic action of drugs is predicated on their physical engagement with cellular targets. Here we describe a broadly applicable method using bioluminescence resonance energy transfer (BRET) to reveal t...
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Article
Open AccessMinimum Information about a Biosynthetic Gene cluster
A wide variety of enzymatic pathways that produce specialized metabolites in bacteria, fungi and plants are known to be encoded in biosynthetic gene clusters. Information about these clusters, pathways and met...
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Article
Identification and characterization of the spiruchostatin biosynthetic gene cluster enable yield improvement by overexpressing a transcriptional activator
Spiruchostatins A and B are members of the FK228-family of natural products with potent histone deacetylase inhibitory activities and antineoplastic activities. However, their production in the wild-type stra...
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Article
Open AccessThe structural basis of an NADP+-independent dithiol oxidase in FK228 biosynthesis
The disulfide bond is unusual in natural products and critical for thermal stability, cell permeability and bioactivity. DepH from Chromobacterium violaceum No. 968 is an FAD-dependent enzyme responsible for cata...
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Article
Genome-guided discovery of diverse natural products from Burkholderia sp.
Burkholderia species have emerged as a new source of diverse natural products. This mini-review covers all of the natural products discovered in recent years from Burkholderia sp. by genome-guide...
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Article
Isolation and characterization of spliceostatin B, a new analogue of FR901464, from Pseudomonas sp. No. 2663
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Article
Extractive fermentation for enhanced production of thailandepsin A from Burkholderia thailandensis E264 using polyaromatic adsorbent resin Diaion HP-20
Thailandepsin A is natural product of Burkholderia thailandensis E264 with potent histone deacetylase inhibitory activities and promising anticancer activities. The titer of thailandepsin A is very low (less than...
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Article
Open AccessThailandepsins are new small molecule class I HDAC inhibitors with potent cytotoxic activity in ovarian cancer cells: a preclinical study of epigenetic ovarian cancer therapy
New treatment strategies are emerging to target DNA damage response pathways in ovarian cancer. Our group has previously shown that the class I biased HDAC inhibitor romidepsin (FK228) induces DNA damage respo...
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Article
Yersinia ironomics: comparison of iron transporters among Yersiniapestis biotypes and its nearest neighbor, Yersinia pseudotuberculosis
Although Yersinia pestis epidemic biovars and Yersinia pseudotuberculosis are recently diverged, highly related species, they cause different diseases via disparate transmission routes. Since iron transport syste...
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Chapter
Hybrid Peptide-Polyketide Natural Products: Biosynthesis and Prospects Towards Engineering Novel Molecules
Polyketides and nonribosomal peptides are two groups of secondary metabolites found in many microorganisms and plants. Biosynthesis and genetic engineering of polyketide and nonribosomal peptide natural produc...
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Chapter
Enzymology, Molecular Genetics, and Regulation of Biosynthesis of the Host-Selective Toxin HC-Toxin
HC-toxin is the critical determinant of virulence and specificity in the interaction between race 1 of Cochliobolus carbonum and maize of genotype hm/hm [21]. HC-toxin is a cyclic peptide of structure cyclo(D-Pro...
