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Article
Open AccessCorrection: Heparanase inhibition as a systemic approach to protect the endothelial glycocalyx and prevent microvascular complications in diabetes
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Article
Open AccessHeparanase inhibition as a systemic approach to protect the endothelial glycocalyx and prevent microvascular complications in diabetes
Diabetes mellitus is a chronic disease which is detrimental to cardiovascular health, often leading to secondary microvascular complications, with huge global health implications. Therapeutic interventions tha...
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Article
Better growth-factor binding aids tissue repair
Enhancing the binding of growth factors to heparan sulfate proteoglycans in the extracellular matrix and on cell surfaces improves wound healing and bone regeneration in mice.
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Article
Open AccessShotgun ion mobility mass spectrometry sequencing of heparan sulfate saccharides
Despite evident regulatory roles of heparan sulfate (HS) saccharides in numerous biological processes, definitive information on the bioactive sequences of these polymers is lacking, with only a handful of nat...
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Article
Open AccessIntrinsic tryptophan fluorescence spectroscopy reliably determines galectin-ligand interactions
Galectins are involved in the regulation of divergent physiological and pathological processes and are increasingly recognized to play important roles in a number of diseases. However, a simple and effective w...
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Article
Open AccessBy-Products of Heparin Production Provide a Diverse Source of Heparin-like and Heparan Sulfate Glycosaminoglycans
Global production of pharmaceutical heparin (Hp) is increasing, and the production process from raw mucosal material results in large amounts of waste by-products. These contain lower sulfated Hp-like and hepa...
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Article
Enhancing the glycosciences toolkit: new GAGs in the lineup
Two cell-based resources producing a broad repertoire of glycosaminoglycan structures will facilitate new applications in the glycosciences field and beyond.
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Article
Open AccessStructure and epitope distribution of heparan sulfate is disrupted in experimental lung hypoplasia: a glycobiological epigenetic cause for malformation?
Heparan sulfate (HS) is present on the surface of virtually all mammalian cells and is a major component of the extracellular matrix (ECM), where it plays a pivotal role in cell-cell and cell-matrix cross-talk...
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Article
Disaccharide compositional analysis of heparan sulfate and heparin polysaccharides using UV or high-sensitivity fluorescence (BODIPY) detection
One of the first steps in characterizing heparan sulfate (HS) and its close relative heparin is to conduct disaccharide composition analysis. This provides an overall picture of the structure of the polysaccha...
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Article
Generating heparan sulfate saccharide libraries for glycomics applications
Natural and semi-synthetic heparan sulfate (HS) saccharide libraries are a valuable resource for investigating HS structure–function relationships, enabling high-throughput glycomics studies. Owing to the diff...
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Protocol
Labelling Heparan Sulphate Saccharides with Chromophore, Fluorescence and Mass Tags for HPLC and MS Separations
The analysis of heparin/HS saccharides derived from small amounts of tissue or cells is a considerable technical challenge and the development of methods to characterise these carbohydrates has progressed comp...
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Protocol
Sequencing Heparan Sulfate Saccharides
The functions of the heparan sulfates (HSs) are determined by specific saccharide motifs within HS chains. These sequences confer selective protein binding properties and the ability to modulate protein activi...
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Protocol
Saccharide Microarrays for High-Throughput Interrogation of Glycan-Protein Binding Interactions
This chapter describes two methods for fabricating microarrays of saccharides for display and interrogation with binding proteins, using fluorescence detection. The first approach is based on the rapid immobil...
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Article
Emerging glycomics technologies
The nascent field of glycomics is currently undergoing rapid development, largely as a result of advances in technologies for analyzing glycan structure, unraveling glycan-protein interactions and establishing...
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Article
Synthetic sugars enhance the functional glycomics toolkit
Glycosaminoglycan-protein interactions are an important frontier for discovering new mechanisms of cellular regulation by complex sugars. The integration of the 'chemical glycomics' strategies of synthetic che...
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Protocol
Sequencing Heparan Sulfate Saccharides
The functions of the heparan sulfates (HSs) are determined by specific saccharide motifs within HS chains. These sequences confer selective protein binding properties and the ability to modulate protein activi...
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Protocol
Integral Glycan Sequencing of Heparan Sulfate and Heparin Saccharides
The functions of heparan sulfate (HS) are determined by specific saccharide motifs within HS chains. These sequences confer selective protein-binding properties and the ability to modulate protein bioactivities (
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Protocol
Analytical and Preparative Strong Anion-Exchange HPLC of Heparan Sulfate and Heparin Saccharides
Studies on the structure-function relationships of the complex linear polysaccha-rides, known as glycosaminoglycans (GAGs), are becoming increasingly important as biological functions are established for them....
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Chapter
Exosequencing of Heparan Sulphate/Heparin Saccharides Using Lysosomal Enzymes
Heparan sulphates (HS) are common components of mammalian cell surfaces and the extracellular matrix. They are normally present in the form of proteoglycans with the HS chains covalently linked to a restricted...
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Protocol
Oligosaccharide Map** and Sequence Analysis of Glycosaminoglycans
A strategy for determining the structure of a glycosaminoglycan (GAG) can be divided into three areas. The first is to establish its saccharide composition. This requires complete depolymerization of the paren...
