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    Article

    Cytokinin oxidase/dehydrogenase inhibitors stimulate 2iP to induce direct somatic embryogenesis in Coffea arabica

    Somatic embryogenesis in Coffea spp. is important for mass production and genetic engineering. Coffee is an exceptional case, as somatic embryogenesis can be induced by applying a cytokinin such as 2iP as the onl...

    Nino Murvanidze, Jaroslav Nisler, Olivier Leroux in Plant Growth Regulation (2021)

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    Chapter

    Cytokinin Properties of Meta-Topolin and Related Compounds

    6-Benzylaminopurine, meta-topolin and their derivatives have been and are being used in and tested for the micropropagation of plants using tissue culture techniques. Meta-topolin and meta-methoxy-topolin were sy...

    Jaroslav Nisler in Meta-topolin: A Growth Regulator for Plant Biotechnology and Agriculture (2021)

  3. Article

    Open Access

    Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum

    Plant hormone cytokinins are perceived by a subfamily of sensor histidine kinases (HKs), which via a two-component phosphorelay cascade activate transcriptional responses in the nucleus. Subcellular localizati...

    Karolina Kubiasová, Juan Carlos Montesinos, Olga Šamajová in Nature Communications (2020)

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    Article

    Characterization of five CHASE-containing histidine kinase receptors from Populus × canadensis cv. Robusta sensing isoprenoid and aromatic cytokinins

    Five poplar CHASE-containing histidine kinase receptors bind cytokinins and display kinase activities. Both endogenous isoprenoid and aromatic cytokinins bind to the receptors in live cell assays.

    Pavel Jaworek, Petr Tarkowski, Tomáš Hluska, Štěpán Kouřil, Ondřej Vrobel in Planta (2019)

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    Article

    Phenolic and flavonoid production and antimicrobial activity of Gymnosporia buxifolia (L.) Szyszyl cell cultures

    Gymnosporia buxifolia (Celastraceae) is a well-known traditional medicinal plant used to treat various diseases. The aim of the study was to quantify the total phenolic and flavonoid content of cell biomass of G...

    Aloka Kumari, Devashan Naidoo, Ponnusamy Baskaran, Karel Doležal in Plant Growth Regulation (2018)

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    Chapter

    TDZ: Mode of Action, Use and Potential in Agriculture

    Strong cytokinin effects of thidiazuron (TDZ) in many plant species have been observed since its discovery in the 1970s. Several of these effects, such as cell division stimulatory activity, anti-senescence, a...

    Jaroslav Nisler in Thidiazuron: From Urea Derivative to Plant Growth Regulator (2018)

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    Article

    Novel thidiazuron-derived inhibitors of cytokinin oxidase/dehydrogenase

    Two new TDZ derivatives (HETDZ and 3FMTDZ) are very potent inhibitors of CKX and are promising candidates for in vivo studies.

    Jaroslav Nisler, David Kopečný, Radka Končitíková in Plant Molecular Biology (2016)

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    Article

    Nebularine Affects Plant Growth and Development but does not Interfere with Cytokinin Signaling

    Nebularine is known for its high cytotoxicity in animals, whereas in plants it was originally believed to be an anticytokinin. In this study we show that in classical cytokinin bioassays, nebularine antagonize...

    Hana Pospíšilová, Jaroslav Nisler, Lukáš Spíchal in Journal of Plant Growth Regulation (2009)