11 Result(s)
within
Jan Bartoš
Plant Sciences
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Article
Open AccessA first survey of the rye (Secale cereale) genome composition through BAC end sequencing of the short arm of chromosome 1R
Rye (Secale cereale L.) belongs to tribe Triticeae and is an important temperate cereal. It is one of the parents of man-made species Triticale and has been used as a source of agronomically important genes for w...
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Chapter
Chromosome Genomics in the Triticeae
The Triticeae species are unique among the important agricultural crops in possessing massive genomes with a prevalence of dispersed DNA repeats. The highest level of complexity is observed in tetraploid and h...
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Chapter and Conference Paper
DArTFest – A Platform for High-Throughput Genome Profiling Within the Festuca – Lolium Complex
With the aim to facilitate high-throughput genome profiling and genetic and physical map** within the Festuca-Lolium complex, we have developed a Diversity Arrays Technology (DArT) array for five important spec...
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Article
Open AccessIntraspecific sequence comparisons reveal similar rates of non-collinear gene insertion in the B and D genomes of bread wheat
Polyploidization is considered one of the main mechanisms of plant genome evolution. The presence of multiple copies of the same gene reduces selection pressure and permits sub-functionalization and neo-functi...
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Article
Open AccessHigh-throughput physical map anchoring via BAC-pool sequencing
Physical maps created from large insert DNA libraries, typically cloned in BAC vector, are valuable resources for map-based cloning and de novo genome sequencing. The maps are most useful if contigs of overlappin...
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Protocol
Localization of Low-Copy DNA Sequences on Mitotic Chromosomes by FISH
Fluorescence in situ hybridization (FISH) is a widely used method to localize DNA sequences on mitotic and meiotic chromosomes and interphase nuclei. It was developed in early 1980s and since then it has contr...
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Article
Frequent occurrence of triploid hybrids Festuca pratensis × F. apennina in the Swiss Alps
The occurrence of triploid hybrids in nature is scarce due to the so-called triploid block representing formation of nonviable progeny after mating diploid with tetraploid. Here we describe frequent presence o...
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Protocol
Generation of Deletion Lines in Allohexaploid Bread Wheat
Positional cloning in bread wheat (Triticum aestivum L.) remains a daunting task because of its large genome, high density of repeats, low recombination rate especially in pericentromeric regions and its allopoly...
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Article
Festuca apennina × F. pratensis triploid hybrids exceed their parents in adaptation to broad-environmental conditions
Occurrence of Festuca apennina De Not. (4 × Fape), F. pratensis Huds. (2 × Fp) and the triploid, sterile hybrid F. apennina × F. pratensis (3 × Fape × Fp) was studied in 12 regions of the Swiss Alps. In total, 19...
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Article
Open AccessScoring the number of B chromosomes in Zea mays L. using droplet digital PCR assay
B chromosomes are classified as dispensable genomic components tolerated by cells, which are transmitted to progeny despite providing no benefit in most cases. They have been observed in over 2800 species of p...
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Article
Open AccessThe early impact of mixed canopies with Norway spruce, European beech and silver fir on a new forest floor
The character of pure or mixed forest canopies and their litterfalls contribute to different forest-floor properties. These organic layers and also subjacent topsoil were studied at three study sites covered b...
