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Article
Open AccessChromosome genomics facilitates the marker development and selection of wheat-Aegilops biuncialis addition, substitution and translocation lines
The annual goatgrass, Aegilops biuncialis is a rich source of genes with considerable agronomic value. This genetic potential can be exploited for wheat improvement through interspecific hybridization to increase...
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Article
Open AccessA pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley
Leaf rust, caused by Puccinia hordei, is one of the most widespread and damaging foliar diseases affecting barley. The barley leaf rust resistance locus Rph7 has been shown to have unusually high sequence and hap...
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Article
Open AccessThe early impact of mixed canopies with Norway spruce, European beech and silver fir on a new forest floor
The character of pure or mixed forest canopies and their litterfalls contribute to different forest-floor properties. These organic layers and also subjacent topsoil were studied at three study sites covered b...
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Article
Open AccessThe wheat stem rust resistance gene Sr43 encodes an unusual protein kinase
To safeguard bread wheat against pests and diseases, breeders have introduced over 200 resistance genes into its genome, thus nearly doubling the number of designated resistance genes in the wheat gene pool1. Iso...
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Article
Open AccessScoring the number of B chromosomes in Zea mays L. using droplet digital PCR assay
B chromosomes are classified as dispensable genomic components tolerated by cells, which are transmitted to progeny despite providing no benefit in most cases. They have been observed in over 2800 species of p...
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Article
Festuca apennina × F. pratensis triploid hybrids exceed their parents in adaptation to broad-environmental conditions
Occurrence of Festuca apennina De Not. (4 × Fape), F. pratensis Huds. (2 × Fp) and the triploid, sterile hybrid F. apennina × F. pratensis (3 × Fape × Fp) was studied in 12 regions of the Swiss Alps. In total, 19...
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Protocol
Generation of Deletion Lines in Allohexaploid Bread Wheat
Positional cloning in bread wheat (Triticum aestivum L.) remains a daunting task because of its large genome, high density of repeats, low recombination rate especially in pericentromeric regions and its allopoly...
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Article
Open AccessPh2 encodes the mismatch repair protein MSH7-3D that inhibits wheat homoeologous recombination
Meiotic recombination is a critical process for plant breeding, as it creates novel allele combinations that can be exploited for crop improvement. In wheat, a complex allohexaploid that has a diploid-like beh...
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Article
Frequent occurrence of triploid hybrids Festuca pratensis × F. apennina in the Swiss Alps
The occurrence of triploid hybrids in nature is scarce due to the so-called triploid block representing formation of nonviable progeny after mating diploid with tetraploid. Here we describe frequent presence o...
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Protocol
Localization of Low-Copy DNA Sequences on Mitotic Chromosomes by FISH
Fluorescence in situ hybridization (FISH) is a widely used method to localize DNA sequences on mitotic and meiotic chromosomes and interphase nuclei. It was developed in early 1980s and since then it has contr...
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Article
Open AccessHigh-throughput physical map anchoring via BAC-pool sequencing
Physical maps created from large insert DNA libraries, typically cloned in BAC vector, are valuable resources for map-based cloning and de novo genome sequencing. The maps are most useful if contigs of overlappin...
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Article
Variation in genome composition of blue-aleurone wheat
Different blue-aleurone wheats display major differences in chromosome composition, ranging from disomic chromosome additions, substitutions, single chromosome arm introgressions and chromosome...
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Article
Open AccessIntraspecific sequence comparisons reveal similar rates of non-collinear gene insertion in the B and D genomes of bread wheat
Polyploidization is considered one of the main mechanisms of plant genome evolution. The presence of multiple copies of the same gene reduces selection pressure and permits sub-functionalization and neo-functi...
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Article
Open AccessChromosomes in the flow to simplify genome analysis
Nuclear genomes of human, animals, and plants are organized into subunits called chromosomes. When isolated into aqueous suspension, mitotic chromosomes can be classified using flow cytometry according to ligh...
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Article
Genetic map** of DArT markers in the Festuca–Lolium complex and their use in freezing tolerance association analysis
Species belonging to the Festuca–Lolium complex are important forage and turf species and as such, have been studied intensively. However, their out-crossing nature and limited availability of molecular markers m...
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Article
Genomic constitution of Festuca × Lolium hybrids revealed by the DArTFest array
Complementary attributes of Festuca and Lolium grasses can be combined in hybrid cultivars called Festuloliums, which are becoming increasingly popular fodder crops and amenity plants. Genomic constitution of com...
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Chapter and Conference Paper
DArTFest – A Platform for High-Throughput Genome Profiling Within the Festuca – Lolium Complex
With the aim to facilitate high-throughput genome profiling and genetic and physical map** within the Festuca-Lolium complex, we have developed a Diversity Arrays Technology (DArT) array for five important spec...
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Article
Open AccessDevelopment and map** of DArT markers within the Festuca - Lolium complex
Grasses are among the most important and widely cultivated plants on Earth. They provide high quality fodder for livestock, are used for turf and amenity purposes, and play a fundamental role in environment pr...
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Chapter
Chromosome Genomics in the Triticeae
The Triticeae species are unique among the important agricultural crops in possessing massive genomes with a prevalence of dispersed DNA repeats. The highest level of complexity is observed in tetraploid and h...
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Article
Open AccessDevelopment of microsatellite markers specific for the short arm of rye (Secale cereale L.) chromosome 1
We developed 74 microsatellite marker primer pairs yielding 76 polymorphic loci, specific for the short arm of rye chromosome 1R (1RS) in wheat background. Four libraries enriched for microsatellite motifs AG,...
