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Article
Open AccessDisordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners
Complex II (CII) activity controls phenomena that require crosstalk between metabolism and signaling, including neurodegeneration, cancer metabolism, immune activation, and ischemia-reperfusion injury. CII act...
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Protocol
Spatial Analysis of Nucleotide Metabolism: From CRISPR Knockout Cancer Cells to MALDI Imaging of Tumors
Cancer cells depend on nucleotides for proliferation. Inhibition of nucleotide metabolism by antimetabolites is a well-established anticancer therapy. However, resistance and toxicity to antimetabolite treatme...
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Article
Open AccessGeneration and characterization of human U-2 OS cell lines with the CRISPR/Cas9-edited protoporphyrinogen oxidase IX gene
In humans, disruptions in the heme biosynthetic pathway are associated with various types of porphyrias, including variegate porphyria that results from the decreased activity of protoporphyrinogen oxidase IX ...
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Protocol
In Vitro Reconstitution of Molecular Motor-Driven Mitochondrial Transport
Intracellular trafficking of organelles driven by molecular motors underlies essential cellular processes. Mitochondria, the powerhouses of the cell, are one of the major cargoes of molecular motors. Efficient...
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Article
SMAD4 loss limits the vulnerability of pancreatic cancer cells to complex I inhibition via promotion of mitophagy
Pancreatic cancer is one of the deadliest forms of cancer, which is attributed to lack of effective treatment options and drug resistance. Mitochondrial inhibitors have emerged as a promising class of anticanc...
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Article
Open AccessMitochondria-adaptor TRAK1 promotes kinesin-1 driven transport in crowded environments
Intracellular trafficking of organelles, driven by kinesin-1 step** along microtubules, underpins essential cellular processes. In absence of other proteins on the microtubule surface, kinesin-1 performs mic...
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Article
Open AccessReplication and ribosomal stress induced by targeting pyrimidine synthesis and cellular checkpoints suppress p53-deficient tumors
p53-mutated tumors often exhibit increased resistance to standard chemotherapy and enhanced metastatic potential. Here we demonstrate that inhibition of dihydroorotate dehydrogenase (DHODH), a key enzyme of th...
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Article
Open AccessSelective elimination of senescent cells by mitochondrial targeting is regulated by ANT2
Cellular senescence is a form of cell cycle arrest that limits the proliferative potential of cells, including tumour cells. However, inability of immune cells to subsequently eliminate senescent cells from th...
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Article
Open AccessAlternative assembly of respiratory complex II connects energy stress to metabolic checkpoints
Cell growth and survival depend on a delicate balance between energy production and synthesis of metabolites. Here, we provide evidence that an alternative mitochondrial complex II (CII) assembly, designated a...
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Chapter
Mitocans: Mitochondrially Targeted Anti-cancer Drugs
Mitochondria are intriguing organelles that are inherently present in most eukaryotic cells, with notable exceptions. They undertake multiple vital functions in a cell, including energy conversion, metabolite ...
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Protocol
Evaluation of Respiration of Mitochondria in Cancer Cells Exposed to Mitochondria-Targeted Agents
Respiration is one of the major functions of mitochondria, whereby these vital organelles use oxygen to produce energy. Many agents that may be of potential clinical relevance act by targeting mitochondria, wh...
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Chapter
Mitochondrial Complex II in Cancer
The mitochondrial connection in cancer has long been suspected, but the first unequivocal genetic link was established only when hereditary paraganglioma-associated mutations were found in genes coding for the...
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Article
K-Ras and mitochondria: Dangerous liaisons
It is well documented that the K-RAS oncogene efficiently transforms non-malignant cells, and there is some evidence for the role of mitochondria in this process. Now Peng Huang and colleagues show that K-Ras ind...
