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Article
An arginine to cysteine252 mutation in insulin receptors from a patient with severe insulin resistance inhibits receptor internalisation but preserves signalling events
Aims/hypothesis. We examined the properties of a mutant insulin receptor (IR) with an Arg252 to Cys (IRR252C) substitution in the α-subunit originally identified in a patient with extreme insulin...
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Article
Epidermal growth factor secretion by submandibular glands is not perturbed in the early phase of streptozotocin-induced diabetes in mice
In rodents, diabetes mellitus is accompanied by a decreased concentration of epidermal growth factor (EGF) in plasma and urine and by a reduced number of EGF receptors on the surface of target cells. A combina...
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Chapter
Regulation of the Na+/K+-pump proteins in skeletal muscle: Acute effects of insulin on α2 distribution and muscle fibre-type specific expression of β subunits
Insulin is a major anabolic hormone that plays a pivotal role in K+ homeostasis. A decrease in plasma K+ concentration is mentioned in the first reports of insulin effects on the whole organism. The hormone enhan...
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Chapter and Conference Paper
Surface Distribution and Pathway of Internalization of C3b Receptors (CR1) in Human Neutrophils
Neutrophils are implicated in the prevention of bacterial infection. To that end, phagocytes must first sense the presence of bacteria, reach the sites of infection and eventually get in close contact with the...
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Article
The cell biology of the insulin receptor
Following initial binding to specific cell surface receptors insulin is internalized in target cells. The fate of the internalized insulin-receptor complexes and how the processes involved are regulated is rev...
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Chapter
Internalization of polypeptide hormones and receptor recycling
The insulin receptor is an integral plasma membrane glycoprotein of most cells. It consists of 2 subunits linked by disulfide bonds; the alpha and beta subunits of the receptor are synthesized by way of a sing...
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Article
Internalization of polypeptide hormones and receptor recycling
The insulin receptor is an integral protein of the plasma membrane of the cell. It is composed of two subunits: an α subunit, which binds the hormone, and a β subunit which is a tyrosine specific protein kinas...
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Article
Quantitative evaluation of gap junctions in rat brown adipose tissue after cold acclimation
The decrease in the metabolic capacity of rat brown adipose tissue during the late postnatal period can be reversed by cold acclimation of the animals. In order to find out whether a parallel decrease in capab...
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Article
Internalization of polypeptide hormones
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Article
Relationship of binding to internalization of125I-insulin in isolated rat hepatocytes
By quantitative electron microscopic autoradiographic technique, we have previously shown that125I-insulin initially localizes to the plasma membrane of isolated rat hepatocytes and is subsequently internalized i...
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Article
125I-Insulin is preferentially internalized in regions of the hepatocytes rich in lysosomal and Golgi structures
125I-Insulin initially localizes to the plasma membrane of isolated rat hepatocytes but is subsequently internalized and preferentially associates with lysosomal structures. In the present study, we show that th...
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Article
An alteration in internodal myelin membrane structure in large sciatic nerve fibres in rats with acute streptozotocin diabetes and impaired nerve conduction velocity
Replicas of the freeze-fracture surfaces of the internodal myelin membranes of large sciatic nerve fibres from normal and diabetic rats were compared by quantitative electron microscopy. The internodal myelin ...
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Article
Glucagon secretion in diabetic patients with idiopathic haemochromatosis
The aim of the present investigation was to determine in patients with idiopathic haemochromatosis whether diabetes is of the primary type or secondary to pancreatic injury due to iron deposition. For this pur...
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Article
Pancreatic polypeptide: A possible role in the regulation of food intake in the mouse. Hypothesis
Pancreatic polypeptide (PP) is a recently identified hormone produced by pancreatic endocrine cells. The islets of genetically obese mice (ob/ob, C57 BL/6J), which are suspected to lack a circulating satiety f...
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Chapter and Conference Paper
Morphologic Study of Glucagon Release
The authors studied the release of glucagon from isolated pancreatic islets using thin section preparations, freeze-fracture techniques and radioimmunoassay. The evidence strongly suggests that exocytosis is a...
