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  1. Article

    Open Access

    Optimization of the production and characterization of milk clotting enzymes by Bacillus subtilis natto

    Suitable medium for production of milk clotting enzyme (MCE) by Bacillus subtilis (natto) Takahashi in submerged liquid-state fermentation was screened, the nutrient factors affecting MCE production was optimized...

    Fang-Chen Wu, Chen-Wei Chang, Ing-Lung Shih in SpringerPlus (2013)

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    Article

    Production of antifungal lipopeptide from Bacillus subtilis in submerged fermentation using shake flask and fermentor

    Optimization of the production of antifungal lipopeptide, iturin A, by B. subtilis was carried out in submerged cultural fermentation. In a shake-flask experiment, response surface methodology (RSM) was employed ...

    Ing-Lung Shih, Chih-Yang Lin, Jane-Yii Wu… in Korean Journal of Chemical Engineering (2009)

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    Article

    Simultaneous and selective production of levan and poly(γ-glutamic acid) by Bacillus subtilis

    Bacillus subtilis(natto) Takahashi, used to prepare the fermented soybean product natto, was grown in a basal medium containing 5% (w/w) sucrose and 1.5% (w/w) l-glutamate and produced 58...

    Ing-Lung Shih, Yun-Ti Yu in Biotechnology Letters (2005)

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    Article

    Antifreeze activities of poly(γ-glutamic acid) produced by Bacillus licheniformis

    Various enantiomeric isomers, metals salts and molecular sizes of poly(γ-glutamic acid), γ-PGA, produced by Bacillus licheniformis CCRC 12826, were prepared and their antifreeze activities were studied by differe...

    Ing-Lung Shih, Yi-Tsong Van, Yi-Yuan Sau in Biotechnology Letters (2003)

  5. No Access

    Article

    A tandem enzymatic hydrolysis of 3-acetylthio-2-methylpropionic methy ester

    Enzymatic hydrolysis of racemic 3-acetylthio-2-methylpropionic methyl ester catalyzed by bovine pancreatic protease and Mucor javanicus lipase showed opposite enantioselecivity. A tandem hydrolysis of the ester c...

    Dar-Fu Tai, Ching-Chong Huang, Hui-Yao Huang, Ing-Lung Shih in Biotechnology Letters (1993)