-
Article
CRISPR/Cas9 using a transient transformation system in Ceriporiopsis subvermispora
Ceriporiopsis subvermispora is a white-rot fungus with great potential for industrial and biotechnological applications, such as the pretreatment of lignocellulose in biorefineries, as it decomposes the lignin in...
-
Article
Open AccessMarker-free genome editing in the edible mushroom, Pleurotus ostreatus, using transient expression of genes required for CRISPR/Cas9 and for selection
In a previous study, we reported a transient transformation system using repeated screening for hygromycin B (Hyg) resistance in the basidiomycete Ceriporiopsis subvermispora. In the present study, by combining t...
-
Article
Influence of ice storage period before freezing on quality of frozen chub mackerel Scomber japonicus fillets and considerations regarding high-quality frozen sashimi products
Achieving high quality levels of frozen chub mackerel Scomber japonicus sashimi products was examined using scientific analysis and sensory evaluation. Frozen fillets stored in ice for different periods before fr...
-
Article
Open AccessEfficient genome editing with CRISPR/Cas9 in Pleurotus ostreatus
Pleurotus ostreatus is one of the most commercially produced edible mushrooms worldwide. Improved cultivated strains with more useful traits have been obtained using classical breeding, which is laborious and ti...
-
Article
Comparative transcriptional analyses of Pleurotus ostreatus mutants on beech wood and rice straw shed light on substrate-biased gene regulation
Distinct wood degraders occupying their preferred habitats have biased enzyme repertoires that are well fitted to their colonized substrates. Pleurotus ostreatus, commonly found on wood, has evolved its own enzym...
-
Chapter
Development of the Bio 3D Printer “Regenova®,” the Robotic System
A unique bio 3D printer has been created using a specific type of scaffolding that can be configured to produce unique tissue configurations. The work described led to the development of an automated bio 3D pr...
-
Article
Open AccessMolecular breeding of sporeless strains of Pleurotus ostreatus using a non-homologous DNA end-joining defective strain
Gene targeting is useful to isolate strains with mutations in a gene of interest for efficient breeding. In this study, we generated msh4 or mer3 single-gene disruptant monokaryons using a Pleurotus ostreatus Δku...
-
Article
Characterization of the effects of terminators and introns on recombinant gene expression in the basidiomycete Ceriporiopsis subvermispora
Terminators and introns are vital regulators of gene expression in many eukaryotes; however, the functional importance of these elements for controlling gene expression in Agaricomycetes remains unclear. In th...
-
Article
Correction to: A 14-bp stretch plays a critical role in regulating gene expression from β1-tubulin promoters of basidiomycetes
The original publication of this paper unfortunately contained three errors in Figs. 2B and 3. In Fig. 2B, the TSS site must be counted as “+ 1” instead of “− 1”. And we indicated wrong sequences in Fig. 3: th...
-
Article
A 14-bp stretch plays a critical role in regulating gene expression from β1-tubulin promoters of basidiomycetes
Cis-acting elements play a vital role in regulation of transcription initiation. Several cis-acting elements have been identified in filamentous fungi; however, the fundamental requirements for basic promoter fun...
-
Article
Open AccessAltered lignocellulose chemical structure and molecular assembly in CINNAMYL ALCOHOL DEHYDROGENASE-deficient rice
Lignin is a complex phenylpropanoid polymer deposited in plant cell walls. Lignin has long been recognized as an important limiting factor for the polysaccharide-oriented biomass utilizations. To mitigate lign...
-
Article
Open AccessOsCAldOMT1 is a bifunctional O-methyltransferase involved in the biosynthesis of tricin-lignins in rice cell walls
Lignin is a phenylpropanoid polymer produced in the secondary cell walls of vascular plants. Although most eudicot and gymnosperm species generate lignins solely via polymerization of p-hydroxycinnamyl alcohols (...
-
Article
Open AccessComparative evaluations of lignocellulose reactivity and usability in transgenic rice plants with altered lignin composition
The aromatic composition of lignins is considered an important trait that affects the physico-chemical properties of lignocellulosic biomass. However, our knowledge of the relationship between lignin structure...
-
Chapter
Cinnamyl Alcohol Dehydrogenase Deficiency Causes the Brown Midrib Phenotype in Rice
Because lignin encrusts lignocellulose polysaccharides, it presents obstacles to chemical pul**, forage digestion, and enzymatic hydrolysis of plant cell wall polysaccharides for biorefining. Hence, it would...
-
Article
Regulation of CONIFERALDEHYDE 5-HYDROXYLASE expression to modulate cell wall lignin structure in rice
Regulation of a gene encoding coniferaldehyde 5-hydroxylase leads to substantial alterations in lignin structure in rice cell walls, identifying a promising genetic engineering target for impro...
-
Article
Comparing the methods for quantifying the culm surface area of bamboo, Phyllostachys pubescens
We compared several methods for quantifying the culm surface area (S) of one of the most common bamboos in Japan, Phyllostachys pubescens Mazel ex Houz. Nine sample culms of P. pubescens were felled, and the true...
-
Article
DSC analysis on water state of salvia hydrogels
The role of the water structure present in hydrogels from nutlets of three species of salvias,S. miltiorrhiza (SM),S. sclarea (SS) andS. viridis (SV), was analyzed by differential scanning calorimetry (DSC). The ...
-
Article
Effects of salts on rheological behaviour of salvia hydrogels
Rheological behavior of natural hydrogel produced from seeds of threeSalvia spp. (S. miltiorrhiza (SM),S. sclarea (SS), S.viridis (SV)) was investigated by using a Rheometer equipped with a cone and plate geometr...
-
Article
PCR-RFLP analysis of the whole chloroplast DNA from three cultivated species of cotton (Gossypium L.)
Variations of the chloroplast DNA (cpDNA) from three of the four cultivated species of cotton (Malvaceae); Gossypium barbadense L., Gossypium hirsutum L., Gossypium arboreum L. and its synonym Gossypium nanking M...
-
Article
Dynamics of human cardiorespiratory responses to standing on one leg with eyes closed
The postural control system has been extensively studied in terms of somatic motor function but little is known about its connection with human autonomic function. The purpose of this study was to determine t...
