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Article
Open AccessNetworked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels
This study evaluated whether cell sheets containing a network of lymphatic endothelial cells (LECs) promoted lymphangiogenesis after transplantation in vivo. Cell sheets with a LEC network were constructed by ...
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Article
Open AccessExternal pressure dynamics promote kidney viability and perfusate filtration during ex vivo kidney perfusion
Normothermic machine perfusion (NMP) has not yet been established as a technique for preserving organs for a day. A key contributing factor to the same is that the perfusing solutions cannot circulate continuo...
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Article
Open AccessBioartificial pulsatile cuffs fabricated from human induced pluripotent stem cell-derived cardiomyocytes using a pre-vascularization technique
There is great interest in the development of techniques to bioengineer pulsatile myocardial tissue as a next-generation regenerative therapy for severe heart failure. However, creation of thick myocardial gra...
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Article
Open AccessCorrection to: Intermittent application of external positive pressure helps to preserve organ viability during ex vivo perfusion and culture
article Intermittent application
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Article
Open AccessIntermittent application of external positive pressure helps to preserve organ viability during ex vivo perfusion and culture
The perfusion of medium through blood vessels allows the preservation of donor organs and culture of bioengineered organs. However, tissue damage due to inadequate perfusion remains a problem. We evaluated whe...
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Article
Open AccessDinaciclib potently suppresses MCL-1 and selectively induces the cell death in human iPS cells without affecting the viability of cardiac tissue
Induced pluripotent stem (iPS) cells hold great potential for being a major source of cells for regenerative medicine. One major issue that hinders their advancement to clinic is the persistence of undifferent...
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Article
Open AccessTubular Cardiac Tissues Derived from Human Induced Pluripotent Stem Cells Generate Pulse Pressure In Vivo
Human induced pluripotent stem (iPS) cell-derived cardiac cells provide the possibility to fabricate cardiac tissues for transplantation. However, it remains unclear human bioengineered cardiac tissues functio...
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Article
Open AccessTRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues
The development of a suitable strategy for eliminating remaining undifferentiated cells is indispensable for the use of human-induced pluripotent stem (iPS) cell-derived cells in regenerative medicine. Here, w...
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Cell Sheet Tissue Engineering for Heart Failure
In recent years, regenerative medicine using cells for treating tissue defects has been in the spotlight as a new treatment for severe heart failure. Direct injection of bone marrow-derived cells and isolated ...
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Article
Open AccessIn Vitro Engineering of Vascularized Tissue Surrogates
In vitro scaling up of bioengineered tissues is known to be limited by diffusion issues, specifically a lack of vasculature. Here, we report a new strategy for preserving cell viability in three-dimensional tissu...
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Article
Open AccessIn vitro fabrication of functional three-dimensional tissues with perfusable blood vessels
Artificially engineered tissues may have many therapeutic applications but complex tissues are hard to create in vitro. Here, Okano and colleagues report the production of functional cardiac tissue sheets with pe...
