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Article
Author Correction: Myt1l safeguards neuronal identity by actively repressing many non-neuronal fates
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Protocol
CRISPR/Cas9 Genome Engineering in Human Pluripotent Stem Cells for Modeling of Neurological Disorders
Recent advances in genome editing have brought new hopes for personalized and precision medicine but have also dramatically facilitated disease modeling studies. Combined with reprogramming approaches, stem ce...
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Protocol
Transcription Factor Programming of Human Pluripotent Stem Cells to Functionally Mature Astrocytes for Monocultures and Cocultures with Neurons
Astrocytes are essential cells for normal brain functionality and have recently emerged as key players in many neurological diseases. However, the limited availability of human primary astrocytes for cell cult...
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Article
Author Correction: Rapid and efficient induction of functional astrocytes from human pluripotent stem cells
In the version of Supplementary Fig. 1 originally published with this paper, some images in panel e were accidental duplicates of images in panel b. This error has been corrected in the online integrated suppl...
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Protocol
In Vitro Functional Characterization of Human Neurons and Astrocytes Using Calcium Imaging and Electrophysiology
Recent progress in stem cell biology and epigenetic reprogramming has opened up previously unimaginable possibilities to study and develop regenerative approaches for neurological disorders. Human neurons and ...
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Article
Open AccessGroucho related gene 5 (GRG5) is involved in embryonic and neural stem cell state decisions
Groucho related gene 5 (GRG5) is a multifunctional protein that has been implicated in late embryonic and postnatal mouse development. Here, we describe a previously unknown role of GRG5 in early developmental...
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Article
Rapid and efficient induction of functional astrocytes from human pluripotent stem cells
The derivation of astrocytes from human pluripotent stem cells is currently slow and inefficient. We demonstrate that overexpression of the transcription factors SOX9 and NFIB in human pluripotent stem cells r...
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Article
Open AccessDirect conversion of human fibroblasts to functional excitatory cortical neurons integrating into human neural networks
Human fibroblasts can be directly converted to several subtypes of neurons, but cortical projection neurons have not been generated.
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Article
Generation of pure GABAergic neurons by transcription factor programming
Transient transcription factor expression rapidly induces a homogenous population of mature GABAergic neurons from human pluripotent stem cells, aiding the study of inhibitory neuron function and disease.
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Article
Myt1l safeguards neuronal identity by actively repressing many non-neuronal fates
The neuron-specific transcription factor Myt1l represses many somatic lineage programs, but not the neuronal lineage program, to both induce and maintain neuronal identity.
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Article
Generation of oligodendroglial cells by direct lineage conversion
Two studies show direct conversion of mouse embryonic fibroblasts to oligodendrocyte progenitor cells capable of generating myelinating oligodendrocytes.
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Protocol
Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain
Neural stem cells are defined as cells that either gives rise to or derives from the cells of the central nervous system and have the unique properties of stem cells, i.e. self-renewal and multipotentiality. O...
