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Article
Open AccessLongitudinal multi-omic changes in the transcriptome and proteome of peripheral blood cells after a 4 Gy total body radiation dose to Rhesus macaques
Non-human primates, such as Rhesus macaques, are a powerful model for studies of the cellular and physiological effects of radiation, development of radiation biodosimetry, and for understanding the impact of ...
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Article
Open AccessMachine learning approach for quantitative biodosimetry of partial-body or total-body radiation exposures by combining radiation-responsive biomarkers
During a large-scale radiological event such as an improvised nuclear device detonation, many survivors will be shielded from radiation by environmental objects, and experience only partial-body irradiation (P...
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Protocol
High-Throughput γ-H2AX Assay Using Imaging Flow Cytometry
The γ-H2AX assay is a sensitive and reliable method to evaluate radiation-induced DNA double-strand breaks. The conventional γ-H2AX assay detects individual nuclear foci manually, but is labor-intensive and ti...
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Article
Open AccessA machine learning method for improving the accuracy of radiation biodosimetry by combining data from the dicentric chromosomes and micronucleus assays
A large-scale malicious or accidental radiological event can expose vast numbers of people to ionizing radiation. The dicentric chromosome (DCA) and cytokinesis-block micronucleus (CBMN) assays are well-establ...
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Article
Open AccessMachine learning methodology for high throughput personalized neutron dose reconstruction in mixed neutron + photon exposures
We implemented machine learning in the radiation biodosimetry field to quantitatively reconstruct neutron doses in mixed neutron + photon exposures, which are expected in improvised nuclear device detonations....
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Article
Open AccessVADER: a variable dose-rate external 137Cs irradiator for internal emitter and low dose rate studies
In the long term, 137Cs is probably the most biologically important agent released in many accidental (or malicious) radiation disasters. It can enter the food chain, and be consumed, or, if present in the enviro...
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Article
Open AccessDevelopment of the FAST-DOSE assay system for high-throughput biodosimetry and radiation triage
Following a large-scale radiological incident, there is a need for FDA-approved biodosimetry devices and biomarkers with the ability to rapidly determine past radiation exposure with sufficient accuracy for ea...
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Article
DNA damage response in peripheral mouse blood leukocytes in vivo after variable, low-dose rate exposure
Environmental contamination and ingestion of the radionuclide Cesium-137 (137Cs) is a large concern in fallout from a nuclear reactor accident or improvised nuclear device, and highlights the need to develop biol...
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Article
Open AccessA High Throughput Approach to Reconstruct Partial-Body and Neutron Radiation Exposures on an Individual Basis
Biodosimetry-based individualized reconstruction of complex irradiation scenarios (partial-body shielding and/or neutron + photon mixtures) can improve treatment decisions after mass-casualty radiation-related...
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Article
Open AccessDevelopment of a high-throughput γ-H2AX assay based on imaging flow cytometry
Measurement of γ-H2AX foci levels in cells provides a sensitive and reliable method for quantitation of the radiation-induced DNA damage response. The objective of the present study was to develop a rapid, hig...
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Article
Open AccessEffect of dose and dose rate on temporal γ-H2AX kinetics in mouse blood and spleen mononuclear cells in vivo following Cesium-137 administration
Cesium-137 (137Cs) is one of the major and most clinically relevant radionuclides of concern in a radiological dispersal device, “dirty bomb” scenario as well as in nuclear accidents and detonations. In this expo...
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Article
Open AccessCandidate protein markers for radiation biodosimetry in the hematopoietically humanized mouse model
After a radiological incident, there is an urgent need for fast and reliable bioassays to identify radiation-exposed individuals within the first week post exposure. This study aimed to identify candidate radi...
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Article
In vitro RABiT measurement of dose rate effects on radiation induction of micronuclei in human peripheral blood lymphocytes
Develo** new methods for radiation biodosimetry has been identified as a high-priority need in case of a radiological accident or nuclear terrorist attacks. A large-scale radiological incident would result i...
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Article
γ-H2AX foci are increased in lymphocytes in vivo in young children 1 h after very low-dose X-irradiation: a pilot study
Computed tomography (CT) is an imaging modality involving ionizing radiation. The presence of γ-H2AX foci after low to moderate ionizing radiation exposure has been demonstrated; however it is unknown whether ...
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Article
The RABiT: high-throughput technology for assessing global DSB repair
At the Center for High-Throughput Minimally Invasive Radiation Biodosimetry, we have developed a rapid automated biodosimetry tool (RABiT); this is a completely automated, ultra-high-throughput robotically ba...
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Article
Modulation of corneal endothelial hydration control mechanisms by Rolipram
Corneal stromal hydration is maintained by an active HCO3 – transport mechanism located in the corneal endothelium. Whilst modulation of transport activity by changes in intracellular cAM...
