14 Result(s)
within
Stephan Mauersberger
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Article
Engineering the α-ketoglutarate overproduction from raw glycerol by overexpression of the genes encoding NADP+-dependent isocitrate dehydrogenase and pyruvate carboxylase in Yarrowia lipolytica
To establish and develop a biotechnological process of α-ketoglutaric acid (KGA) production by Yarrowia lipolytica, it is necessary to increase the KGA productivity and to reduce the amounts of by-products, e.g. ...
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Cytochrome P450 Expression in Yarrowia lipolytica and Its Use in Steroid Biotransformation
This review is a first attempt to systematize data on the potential of transgenic Y. lipolytica to catalyze diverse reactions of steroid transformation. The yeast Y. lipolytica was tested as host for P450-catalyz...
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Cytochromes P450 of the Alkane-Utilising Yeast Yarrowia lipolytica
Since the first studies in 1980s, P450 has been shown to be induced during growth of Y. lipolytica on alkanes, fatty alcohols and fatty acids and evidenced to be involved in terminal hydroxylation of alkanes and ...
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Article
Open AccessSteroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes
Yarrowia lipolytica efficiently metabolizes and assimilates hydrophobic compounds such as n-alkanes and fatty acids. Efficient substrate uptake is enabled by naturally secreted emulsifiers and a modified cell sur...
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Article
Variation of the by-product spectrum during α-ketoglutaric acid production from raw glycerol by overexpression of fumarase and pyruvate carboxylase genes in Yarrowia lipolytica
The yeast Yarrowia lipolytica secretes high amounts of various organic acids, like citric, isocitric, pyruvic (PA), and α-ketoglutaric (KGA) acids, triggered by growth limitation and excess of carbon source. This...
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Aconitase overexpression changes the product ratio of citric acid production by Yarrowia lipolytica
The yeast Yarrowia lipolytica secretes high amounts of various organic acids, like citric acid (CA) and isocitric acid (ICA) under an excess of carbon source and several conditions of growth limitation. Depending...
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Article
Overexpression of the ICL1 gene changes the product ratio of citric acid production by Yarrowia lipolytica
The yeast Yarrowia lipolytica secretes high amounts of various organic acids, like citric (CA) and isocitric (ICA) acids, triggered by growth limitation caused by different factors and an excess of carbon source....
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Article
Citric acid production from sucrose using a recombinant strain of the yeast Yarrowia lipolytica
The yeast Yarrowia lipolytica is able to secrete high amounts of several organic acids under conditions of growth limitation and carbon source excess. Here we report the production of citric acid (CA) in a fed-ba...
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Chapter
Tagging of Genes by Insertional Mutagenesis in the Yeast Yarrowia lipolytica
The aims of the experiments are to obtain tagged mutants in the alkane-uti-lizing yeast Yarrowia lipolytica, to characterize their phenotypes, and to identify the mutated genes by PCR walking methods.
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Induction of Citric Acid/lsocitric Acid and α-Ketoglutaric Acid Production in the Yeast Yarrowia lipolytica
To induce the overproduction of citric and isocitric acids at different proportions, as well as a-ketoglutaric acid, in Y. lipolytica by changing the cultivation conditions.
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Article
Comparison of promoters suitable for regulated overexpression of β-galactosidase in the alkane-utilizing yeastYarrowia lipolytica
Promoters of the genesG3P, ICL1, POT1, POX1, POX2 andPOX5 of the yeastY. lipolytica were studied in respect to their regulations and activities during growth on different carbon sources. The aim of this study was...
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Chapter
Candida maltosa
More than 30 years have passed since the first description of the yeast species Candida (C.) maltosa by Komagata et al. (1964a,b). Since then, C. maltosa has become of considerable academic and commercial interes...
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Article
Substrate specificity and stereoselectivity of fatty alcohol oxidase from the yeast Candida maltosa
In Candida maltosa and other alkene-utilizing yeasts a membrane-bound fatty alcohol oxidase (FAOD) is induced by growth on n-alkenes. The oxidation of 1-alkanols to the corresponding aldehydes is accompanied by t...
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The induction of cytochrome P-450 in the alkane-utilizing yeast Lodderomyces elongisporus: Alterations in the microsomal membrane fraction
The adaptation of Lodderomyces elongisporus cells to n-alkane utilization was found to be connected with several alterations in the enzyme pattern of the whole cell and the microsomal fraction in particular. A st...
