10 Result(s)
within
Frank Stahl
Life Sciences
-
Protocol
Primary and Stem Cell Microarrays: Application as Miniaturized Biotesting Systems
The deposition of living cells on microarray surfaces can be used to create physiologically relevant architecture in vitro. Such living cell microarrays enable the reconstruction of biological processes outsid...
-
Article
Entwicklung komplexer Screening verfahren am Beispiel von Hsp90
The protein target-microarray assay is a hitherto underestimated method in the investigation of protein-ligand/inhibitor and protein-protein/inhibitor-interactions. It is a versatile and adaptable method that ...
-
Chapter
Isolation of Intact RNA from Sorted S. cerevisiae Cells for Differential Gene Expression Analysis
Individuals within natural populations are highly diverse. They can vary regarding their physiological states and thus contribute differently to the performance of the whole community [1]. Hence, the character...
-
Article
Open AccessRegulation of lipid metabolism-related gene expression in whole blood cells of normo- and dyslipidemic men after fish oil supplementation
Beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on the lipid levels of dyslipidemic subjects are widely described in the literature. However, the underlying molecular mechanisms are large...
-
Article
Open AccessDifferent gene expression profiles in normo- and dyslipidemic men after fish oil supplementation: results from a randomized controlled trial
Epidemiological studies have suggested the benefits of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on cardiovascular health, but only limited data are available describing n-3 PUFA regulated pathways in hu...
-
Article
Anwendungen von Aptameren in der Mikroarray-Technologie
Die Anwendung von Aptameren in der Mikroarray-Technik ist eine neue, vielversprechende Methode zur Detektion von Proteinen. Aptamer-Mikroarrays können zudem als Screening-Tools für die Untersuchung der Aptamer...
-
Article
Growth and Differentiation Properties of Mesenchymal Stromal Cell Populations Derived from Whole Human Umbilical Cord
Up to 2.8 × 107 fibroblast-like cells displaying an abundant presence of mesenchymal stem cell (MSC) markers CD73, CD90, CD105 and a low level of HLA-I expression can be isolated from one whole human umbilical co...
-
Article
Isolation of intact RNA from cytometrically sorted Saccharomyces cerevisiae for the analysis of intrapopulation diversity of gene expression
Characterizing and understanding the functional heterogeneity in a given population on the cellular and molecular level is a great challenge in microbiology. Each microorganism contributes differently to the o...
-
Article
Na+-dependent HCO 3 − transport and Na+/H+ exchange regulate pH i in human ciliary muscle cells
We investigated intracellular pH (pH i ) regulation in cultured human ciliary muscle cells by means of the pH-sensitive absorbance of 5(and 6)-carboxy-4′,5′-dimethylfluorescein (...
-
Article
Regulation of cytoplasmic pH of cultured bovine corneal endothelial cells in the absence and presence of bicarbonate
Intracellular pH (pH i ) in confluent monolayers of cultured bovine corneal endothelial cells was determined using the pH-dependent absorbance of intracellularly trapped 5(and 6)...
