-
Article
Open AccessLow impact of regular PCR testing on presence at work site during the COVID-19 pandemic: experiences during an open observational study in Lower Saxony 2020-21
Since social distancing during the COVID-19-pandemic had a profound impact on professional life, this study investigated the effect of PCR testing on on-site work.
-
Article
Open AccessLung Surfactant Accelerates Skin Wound Healing: A Translational Study with a Randomized Clinical Phase I Study
Lung surfactants are used for reducing alveolar surface tension in preterm infants to ease breathing. Phospholipid films with surfactant proteins regulate the activity of alveolar macrophages and reduce inflam...
-
Chapter
Aptazymes: Expanding the Specificity of Natural Catalytic Nucleic Acids by Application of In Vitro Selected Oligonucleotides
Aptazymes are synthetic molecules composed of an aptamer domain and a catalytic active nucleic acid unit, which may be a ribozyme or a DNAzyme. In these constructs the aptamer domain serves as a molecular swit...
-
Chapter
Spezielle labortechnische Reaktoren: Lab-on-a-Chip
Mikroreaktorsysteme ermöglichen es, auf kleinstem Raum verschiedene Prozessschritte kombiniert ablaufen zu lassen. In diesem Kapitel werden die einzelnen funktionellen Einheiten solcher mikrofluidischen System...
-
Article
Open AccessMicroarray-based screening system identifies temperature-controlled activity of Connexin 26 that is distorted by mutations
Here, we show that human Connexin 26 (hCx26 or Cx26WT) hemichannel opening rapidly enables the transport of small molecules when triggered by temperature and by compensation of the Ca2+ blockade with EDTA. Point ...
-
Living Reference Work Entry In depth
Spezielle labortechnische Reaktoren: Lab-on-a-Chip
Mikroreaktorsysteme ermöglichen es auf kleinstem Raum verschiedene Prozessschritte kombiniert ablaufen zu lassen. In diesem Kapitel werden die einzelnen funktionellen Einheiten solcher mikrofluidischen Systeme...
-
Protocol
Primary and Stem Cell Microarrays: Application as Miniaturized Biotesting Systems
The deposition of living cells on microarray surfaces can be used to create physiologically relevant architecture in vitro. Such living cell microarrays enable the reconstruction of biological processes outsid...
-
Article
Entwicklung komplexer Screening verfahren am Beispiel von Hsp90
The protein target-microarray assay is a hitherto underestimated method in the investigation of protein-ligand/inhibitor and protein-protein/inhibitor-interactions. It is a versatile and adaptable method that ...
-
Chapter and Conference Paper
Experimental investigation of the cold start behavior of different coolant circuits for a waste heat recovery system and their influence on the engine
One of the most promising technologies to increase efficiency and to decrease CO2-emissions for future combustion engines is the utilization of exhaust gas heat through a Rankine cycle. A so called waste heat rec...
-
Article
In vitro toxicological nanoparticle studies under flow exposure
The use of nanoparticles is becoming increasingly common in industry and everyday objects. Thus, extensive risk management concerning the potential health risk of nanoparticles is important. Currently, in vitr...
-
Chapter
Molecular Survival Strategies of Organisms: HSP and Small Molecules for Diagnostics and Drug Development
Nature has developed evolutionary strategies to synthesize the vast number of what are called secondary metabolites or in short natural products. These include molecules that are able to inhibit heat shock pro...
-
Chapter
Aptamer-Modified Nanoparticles as Biosensors
Aptamers are short oligonucleotides that are capable of selectively binding to their corresponding target. Therefore, they can be thought of as a nucleic acid-based alternative to antibodies and can substitute...
-
Chapter
Isolation of Intact RNA from Sorted S. cerevisiae Cells for Differential Gene Expression Analysis
Individuals within natural populations are highly diverse. They can vary regarding their physiological states and thus contribute differently to the performance of the whole community [1]. Hence, the character...
-
Article
Open AccessRegulation of lipid metabolism-related gene expression in whole blood cells of normo- and dyslipidemic men after fish oil supplementation
Beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on the lipid levels of dyslipidemic subjects are widely described in the literature. However, the underlying molecular mechanisms are large...
-
Article
Open AccessDifferent gene expression profiles in normo- and dyslipidemic men after fish oil supplementation: results from a randomized controlled trial
Epidemiological studies have suggested the benefits of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on cardiovascular health, but only limited data are available describing n-3 PUFA regulated pathways in hu...
-
Article
Open AccessTranscriptome-based identification of antioxidative gene expression after fish oil supplementation in normo- and dyslipidemic men
The beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs), especially in dyslipidemic subjects with a high risk of cardiovascular disease, are widely described in the literature. A lot of effec...
-
Chapter and Conference Paper
A Comparative Study of Suspension Cultivation Systems for the Expansion of Undifferentiated Mouse Embryonic Stem Cells
Embryonic Stem (ES) cells are self- renewing and pluripotent cells that can differentiate into a variety of cell lineages. Implementation in tissue engineering or as model systems for drug discovery makes ES c...
-
Chapter and Conference Paper
A New Variant of the Affinity Matrix Method for Identification of High Producing Cells in Mammalian Cell Culture
The screening of high-producing cells is a time consuming procedure. Starting from a single cell after a single cell dilution the procedure takes weeks to complete. The affinity matrix method is a technique fo...
-
Chapter
Transcriptome Analysis
Transcriptome analysis technologies are important systems-biology methods for the investigation and optimization of mammalian cell cultures concerning with regard to growth rates and productivity. For the prod...
-
Article
Anwendungen von Aptameren in der Mikroarray-Technologie
Die Anwendung von Aptameren in der Mikroarray-Technik ist eine neue, vielversprechende Methode zur Detektion von Proteinen. Aptamer-Mikroarrays können zudem als Screening-Tools für die Untersuchung der Aptamer...
