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Article
Impact of oxygen supply on rtPA expression in Escherichia coli BL21 (DE3): ammonia effects
In shake flasks, good oxygen supply tended to decrease rtPA expression in media containing only yeast extract and tryptone, while oxygen limitation would increase rtPA synthesis in the same medium. Our data sh...
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Article
Open AccessSafety and pharmacokinetics of novel selective vascular endothelial growth factor receptor-2 inhibitor YN968D1 in patients with advanced malignancies
YN968D1 (Apatinib) selectively inhibits phosphorylation of VEGFR-2 and tumor angiogenesis in mice model. The study was conducted to determine the maximum tolerated dose (MTD), safety profile, pharmacokinetic v...
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Article
A New Steroid-Transforming Strain of Mycobacterium neoaurum and Cloning of 3-Ketosteroid 9α-Hydroxylase in NwIB-01
Using enrichment procedures, five strains that can utilize soybean phytosterols as the sole carbon source were isolated from steroids-contaminated soil samples. Among the isolated strains, the strain NwIB-01 w...
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Article
Knockdown of NtMed8, a Med8-like gene, causes abnormal development of vegetative and floral organs in tobacco (Nicotiana tabacum L.)
Med8, a subunit of mediator complex, has proved to possess crucial functions in many organisms from yeast to human. In plant, the med8 mutant of Arabidopsis thaliana displayed delayed anthesis and increased numbe...
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Article
Identification and expression analysis of Rehmannia glutinosa mediator complex genes in response to continuous crop**
The mediator complex, a conserved assembly of multi-functional proteins, is required for the transcription of almost all protein-encoding genes in eukaryotes. In this report, 36 mediator complex subunit transc...
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Article
Open AccessAssaying the potential autotoxins and microbial community associated with Rehmannia glutinosa replant problems based on its ‘autotoxic circle’
This study attempted to elucidate the underlying correlations among replant problems of Rehmannia glutinosa, the autotoxins and microbes within its root-zone soil.
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Article
Open AccessSafety, pharmacokinetics, and antitumor properties of anlotinib, an oral multi-target tyrosine kinase inhibitor, in patients with advanced refractory solid tumors
Anlotinib is a novel multi-target tyrosine kinase inhibitor that is designed to primarily inhibit VEGFR2/3, FGFR1-4, PDGFR α/β, c-Kit, and Ret. We aimed to evaluate the safety, pharmacokinetics, and antitumor ...
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Chapter and Conference Paper
Preparation and DSC Analysis of γ-Poly Glutamic Acid Ester
With the increasing utilization of plastic in packaging industry, plastic waste caused great impact on the environment. So the research and development of new materials of biodegradable plastics could alleviat...
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Article
A composite monolithic column fabricated with functionalized nanodiamond and its application in separation of small molecules
A nanodiamond-based composite monolithic column was fabricated by redox initiation for high performance liquid chromatography (HPLC). The functionalized nanodiamond was modified via Fenton Reagent and 3-methac...
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Article
Transcriptome analysis reveals metabolic alteration due to consecutive monoculture and abiotic stress stimuli in Rehamannia glutinosa Libosch
We deeply investigated the mechanism underlying metabolic regulation in response to consecutive monoculture (replanting disease) and different abiotic stresses that unfolded the respon...
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Article
Open AccessDifferential proteomic analysis of replanted Rehmannia glutinosa roots by iTRAQ reveals molecular mechanisms for formation of replant disease
The normal growth of Rehmannia glutinosa, a widely used medicinal plant in China, is severely disturbed by replant disease. The formation of replant disease commonly involves interactions among plants, allelochem...
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Article
Open AccessNovel Application of Magnetic Protein: Convenient One-Step Purification and Immobilization of Proteins
Recently, a magnetic protein was discovered, and a multimeric magnetosensing complex was validated, which may form the basis of magnetoreception. In this study, the magnetic protein was firstly used in biotech...
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Article
Open AccessPolymorphism and plasma levels of apolipoprotein E and the risk of aneurysmal subarachnoid hemorrhage in a Chinese population: a case-control study
Aneurysmal subarachnoid hemorrhage (aSAH) is the most common types of subarachnoid hemorrhage, which is a critical clinical problem with high morbidity, mortality, and economic impact. Recent studies have show...
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Article
Open AccessIdentification of Differentially Expressed Non-coding RNA in Porcine Alveolar Macrophages from Tongcheng and Large White Pigs Responded to PRRSV
Porcine reproductive and respiratory syndrome (PRRS) is one of the most ruinous diseases in pig production. Our previous work showed that Tongcheng pigs (TC) were less susceptible to PRRS virus (PRRSV) than La...
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Article
The preparation and therapeutic roles of scFv-Fc antibody against Staphylococcus aureus infection to control bovine mastitis
Staphylococcus aureus–induced bovine mastitis causes significant losses to the dairy industry and available vaccines do not confer adequate protection. As a more attractive alternative, we propose the use of anti...
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Article
Identification of phytotoxic metabolites released from Rehmannia glutinosa suggest their importance in the formation of its replant problem
The yield and quality of medicinal tubers of Rehmannia glutinosa are seriously restricted by replanting problems. However, the origin and identification of the potential allelochemicals associated with this issue...
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Article
Open AccessPlasticity without dislocations in a polycrystalline intermetallic
Dislocation activity is critical to ductility and the mechanical strength of metals. Dislocations are the primary drivers of plastic deformation, and their interactions with each other and with other microstru...
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Article
Metabolic engineering of an industrial Aspergillus niger strain for itaconic acid production
Itaconic acid is a value-added organic acid that is widely applied in industrial production. It can be converted from citric acid by some microorganisms including Aspergillus terreus and Aspergillus niger. Becaus...
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Article
Characterization of a novel halotolerant esterase from Chromohalobacter canadensis isolated from salt well mine
A esterase gene was characterized from a halophilic bacterium Chromohalobacter canadensis which was originally isolated from a salt well mine. Sequence analysis showed that the esterase, named as EstSHJ2, contain...
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Article
Efficient CRISPR/Cas9-mediated genome editing in Rehmannia glutinosa
Here, we cloned a phytoene desaturase (PDS) gene from Rehmannia glutinosa, and realized RgPDS1 knock out in R. glutinosa resulted in the generation of albino plants.
