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  1. No Access

    Article

    Direct sequencing of Arabidopsis thaliana RNA reveals patterns of cleavage and polyadenylation

    RNA 3′-end formation is thought to be crucial in controlling gene expression. Direct RNA sequencing was used to analyze the 3′ ends of Arabidopsis thaliana RNA in unprecedented detail, revealing extreme heterogen...

    Alexander Sherstnev, Céline Duc, Christian Cole… in Nature Structural & Molecular Biology (2012)

  2. Article

    Open Access

    Erratum to: The majority of total nuclear-encoded non-ribosomal RNA in a human cell is 'dark matter' un-annotated RNA

    Philipp Kapranov, Georges St Laurent, Tal Raz, Fatih Ozsolak… in BMC Biology (2011)

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  3. Article

    Open Access

    The majority of total nuclear-encoded non-ribosomal RNA in a human cell is 'dark matter' un-annotated RNA

    Discovery that the transcriptional output of the human genome is far more complex than predicted by the current set of protein-coding annotations and that most RNAs produced do not appear to encode proteins ha...

    Philipp Kapranov, Georges St Laurent, Tal Raz, Fatih Ozsolak… in BMC Biology (2010)

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  4. No Access

    Article

    Amplification-free digital gene expression profiling from minute cell quantities

    On-flowcell capture and reverse transcription followed by single-molecule cDNA sequencing provides reproducible digital gene expression results from as few as 1,000 cells.

    Fatih Ozsolak, David T Ting, Ben S Wittner, Brian W Brannigan… in Nature Methods (2010)

  5. No Access

    Article

    Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA

    Single-molecule sequencing of poly(A)-tailed chromatin immunoprecipitated DNA proves equal in sensitivity and accuracy to amplification-based sequencing technologies and allows analysis of samples sizes as sma...

    Alon Goren, Fatih Ozsolak, Noam Shoresh, Manching Ku, Mazhar Adli… in Nature Methods (2010)

  6. No Access

    Article

    High-throughput map** of the chromatin structure of human promoters

    Our understanding of how chromatin structure influences cellular processes such as transcription and replication has been limited by a lack of nucleosome-positioning data in human cells. We describe a high-res...

    Fatih Ozsolak, Jun S Song, X Shirley Liu, David E Fisher in Nature Biotechnology (2007)

  7. Article

    Open Access

    Comparison of dot chromosome sequences from D. melanogaster and D. virilisreveals an enrichment of DNA transposon sequences in heterochromatic domains

    Chromosome four of Drosophila melanogaster, known as the dot chromosome, is largely heterochromatic, as shown by immunofluorescent staining with antibodies to heterochromatin protein 1 (HP1) and histone H3K9me. I...

    Elizabeth E Slawson, Christopher D Shaffer, Colin D Malone, Wilson Leung… in Genome Biology (2006)

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  8. No Access

    Chapter

    mRNA Applications

    Fatih Ozsolak, Veronica Luzzi, Mark A. Watson in Dissecting the Molecular Anatomy of Tissue (2005)