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Chapter and Conference Paper
Natural Variation And Identification Of Microelements Content In Seeds Of Einkorn Wheat (Triticum Monococcum)
Micronutrient deficiencies in human beings are common problems, especially in develo** world. Among the micronutrient deficiencies, zinc (Zn) and iron (Fe) deficiencies are particularly important affecting s...
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Article
Identification of further Craterostigma plantagineum cdt mutants affected in abscisic acid mediated desiccation tolerance
The resurrection plant (Craterostigma plantagineum) is desiccation tolerant. However, callus derived from this plant, when propagated in vitro, requires exogenously applied abscisic acid (ABA) in order to survive...
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Article
A reconsideration of the domestication geography of tetraploid wheats
The domestication of tetraploid wheats started from their wild progenitor Triticum dicoccoides. In this paper, the geographical distribution of this progenitor is revised to include more sampling locations. The p...
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Article
Structural diversity and organization of three gene families for Kunitz-type enzyme inhibitors from potato tubers ( Solanum tuberosum L.)
In the potato, Kunitz-type enzyme inhibitors are abundant and highly polymorphic small proteins found in tubers. DNA sequence analysis of 1596 unselected ESTs (expressed sequence tags) from mature tubers of th...
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Article
Functional comparison of homologous members of three groups of Kunitz-type enzyme inhibitors from potato tubers ( Solanum tuberosum L.)
For functional studies, nine cDNAs encoding Kunitz-type enzyme inhibitors from potato tubers were expressed as GST (glutathione S transferase)-tagged fusion proteins in the fission yeast Schizosaccharomyces pombe
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Article
Integration of a barley (Hordeum vulgare) molecular linkage map with the position of genetic loci hosting 29 developmental mutants
The first step in positional gene cloning is the integration into available molecular maps of genetic loci for which mutant alleles exist. We report the placement of 29 barley developmental mutants on a restri...
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Article
Members of the Kunitz-type protease inhibitor gene family of potato inhibit soluble tuber invertase in vitro
A soluble proteinaceous invertase inhibitor was purified from tubers of cv. Provita. N-terminal amino acid sequencing of the purified protein indicated that the invertase inhibitor was a member of a family of ...
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Article
Reverse genetic approaches in plants and yeast suggest a role for novel, evolutionarily conserved, selenoprotein-related genes in oxidative stress defense
Oxidation of methionine residues during periods of oxidative stress can lead to loss of protein function. Organisms have developed defense strategies to minimize such damage. The PilB protein, which is involv...
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Article
Map** QTLs for sucrose content, yield and quality in a sugar beet population fingerprinted by EST-related markers
Seventy five expressed sequence tags (ESTs) that are associated with functions in carbohydrate and nitrogen metabolism were genotyped in 108 plants of an F2 population of sugar beet (Beta vulgaris L.) segregating...
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Article
A potato molecular-function map for carbohydrate metabolism and transport
Molecular-linkage maps based on functional gene markers (molecular-function maps) are the prerequisite for a candidate-gene approach to identify genes responsible for quantitative traits at the molecular level...
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Chapter
Genetic Transformation of Craterostigma plantagineum
Dehydration is one of the most common environmental stresses to which plants are exposed, and in many regions it is the bottleneck of agricultural development (McKersie and Leshem 1994). Plant growth is seriou...
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Article
Identification of Photosynthetic Mutants of Arabidopsis by Automatic Screening for Altered Effective Quantum Yield of Photosystem 2
Quantification of chlorophyll (Chl) fluorescence is a versatile tool for analysing the photosynthetic performance of plants in a non-intrusive manner. A pulse-amplitude modulated fluorometer was combined with ...
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Article
PCR-based cloning and segregation analysis of functional gene homologues in Beta vulgaris
To analyse genetic factors that potentially affect sugar quality and yield in Beta vulgaris, we designed primers based on 18 homologous ESTs and conserved regions of 32 heterologous ESTs encoding gene products t...
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Article
Localization of QTLs for tolerance to Cercospora beticola on sugar beet linkage groups
We present a new linkage map for sugar beet (Beta vulgaris) which has been developed using a population segregating for genetic factors that confer tolerance to the leaf spot fungus (Cercospora beticola), the cau...
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Article
TMV resistance gene N homologues are linked to Synchytrium endobioticum resistance in potato
The fungus Synchytrium endobioticum, the causal agent of potato wart disease, is subject to world-wide quarKantine regulations due to the production of persistent resting spores and lack of effective chemical co...
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Chapter
Where do we go from this point
With the rediscovery of Mendel’s laws in 1900, radical changes took place in plant breeding methods. The new science of genetics contributed new concepts: the gene, the role of chromosomes as gene carriers, ge...
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Article
Analysis of quantitative trait loci (QTLs) and quantitative trait alleles (QTAs) for potato tuber yield and starch content
Using RFLP markers, QTLs for tuber starch-content and tuber yield were mapped in two F1 populations derived from crossing non-inbred di-haploid potato breeding lines. QTLs were identified and mapped, based on bo...
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Article
Investigating the molecular basis of desiccation tolerance using the resurrection plant Craterostigma plantagineum as an experimental system
The array of genes isolated and characterised from Craterostigma to date allow many insights to be made into the protection mechanisms underlying the desiccation tolerance phenomenon and drought-induced changes i...
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Article
Construction of a potato YAC library and identification of clones linked to the disease resistance loci R1 and Gro1
A yeast artificial chromosome (YAC) library was constructed from high-molecular-weight DNA of potato (Solanum tuberosum). Potato DNA fragments obtained after complete digestion with four different rare-cutter re...
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Article
The role of multiple binding sites in the activation of zein gene expression byOpaque-2
Opaque-2 (O2) encodes a transcriptional activator of the basic domain-leucine zipper (bZIP) class, which controls the expression level in maize endosperm of the 22 kD α-zeins and a number of non-storage proteins...
