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Article
A kinase-cGAS cascade to synthesize a therapeutic STING activator
The introduction of molecular complexity in an atom- and step-efficient manner remains an outstanding goal in modern synthetic chemistry. Artificial biosynthetic pathways are uniquely able to address this chal...
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Article
N-Glycosylamine-mediated isotope labeling for mass spectrometry-based quantitative analysis of N-linked glycans
N-Linked glycosylation is a major protein modification involved in many essential cellular functions. Methods capable of quantitative glycan analysis are highly valuable and have been actively pu...
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Protocol
High-Throughput Multimodal Strong Anion Exchange Purification and N-Glycan Characterization of Endogenous Glycoprotein Expressed in Glycoengineered Pichia pastoris
The secretory pathway of the yeast Pichia pastoris has been engineered to produce complex human-type N-glycans (Choi et al., Proc Natl Acad Sci USA 100:5022–5027, 2003; Hamilton et al., Science 301:1244–1246, 200...