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Protocol
Liquid Glycan Array
The M13 phage platform is a stable and monodisperse nanoscale carrier, which can be modified with different molecules by chemical conjugation strategies. Here, we describe M13 phage acylated on pVIII protein w...
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Article
Open AccessChemoenzymatic synthesis of genetically-encoded multivalent liquid N-glycan arrays
Cellular glycosylation is characterized by chemical complexity and heterogeneity, which is challenging to reproduce synthetically. Here we show chemoenzymatic synthesis on phage to produce a genetically-encode...
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Article
Genetically encoded multivalent liquid glycan array displayed on M13 bacteriophage
The central dogma of biology does not allow for the study of glycans using DNA sequencing. We report a liquid glycan array (LiGA) platform comprising a library of DNA ‘barcoded’ M13 virions that display 30–1,5...
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Article
Open AccessImpact of sequencing depth and technology on de novo RNA-Seq assembly
RNA-Seq data is inherently nonuniform for different transcripts because of differences in gene expression. This makes it challenging to decide how much data should be generated from each sample. How much shoul...
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Article
Open AccessData access for the 1,000 Plants (1KP) project
The 1,000 plants (1KP) project is an international multi-disciplinary consortium that has generated transcriptome data from over 1,000 plant species, with exemplars for all of the major lineages across the Viridi...
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Article
Correction: Corrigendum: Independent optical excitation of distinct neural populations
Nat. Methods 11, 338–346 (2014); published online 9 February 2014; corrected after print 28 August 2014 In the version of this article initially published, the Drosophila transgenic strains were incorrectly re...
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Article
Addendum: Independent optical excitation of distinct neural populations
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Article
Independent optical excitation of distinct neural populations
Sequencing the transcriptomes of more than 100 species of alga yields new channelrhodopsins with promising properties for optogenetics. A far red–shifted channelrhodopsin, Chrimson, opens up new behavioral cap...
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Article
Open AccessA genome triplication associated with early diversification of the core eudicots
Although it is agreed that a major polyploidy event, gamma, occurred within the eudicots, the phylogenetic placement of the event remains unclear.
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Article
Homology modeling of tubulin: influence predictions for microtubule’s biophysical properties
Using comparative modeling, we have generated structural models of 475 α and β tubulins. Using these models, we observed a global, structural similarity between the tubulin isotypes. However, a number of subtl...
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