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Article
Open AccessAberrant NOVA1 function disrupts alternative splicing in early stages of amyotrophic lateral sclerosis
Amyotrophic lateral sclerosis (ALS) is a fatal disease characterized by aberrant alternative splicing (AS). Nuclear loss and cytoplasmic accumulation of the splicing factor TDP-43 in motor neurons (MN) are hal...
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Article
Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors
Genetic screens using pooled CRISPR-based approaches are scalable and inexpensive, but restricted to standard readouts, including survival, proliferation and sortable markers. However, many biologically releva...
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Article
Open AccessPrinciples of RNA processing from analysis of enhanced CLIP maps for 150 RNA binding proteins
A critical step in uncovering rules of RNA processing is to study the in vivo regulatory networks of RNA binding proteins (RBPs). Crosslinking and immunoprecipitation (CLIP) methods enable map** RBP targets ...
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Article
Transcriptome–pathology correlation identifies interplay between TDP-43 and the expression of its kinase CK1E in sporadic ALS
Sporadic amyotrophic lateral sclerosis (sALS) is the most common form of ALS, however, the molecular mechanisms underlying cellular damage and motor neuron degeneration remain elusive. To identify molecular si...
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Article
RNA-binding protein CPEB1 remodels host and viral RNA landscapes
The RNA-binding protein CPEB1 drives post-transcriptional changes in the host transcriptome and poly(A)-tail lengthening of viral RNAs, processes essential for productive HCMV infection.
