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Article
Open AccessIsland plants with newly discovered reproductive traits have higher capacity for uniparental reproduction, supporting Baker’s law
Uniparental reproduction is advantageous when lack of mates limits outcrossing opportunities in plants. Baker’s law predicts an enrichment of uniparental reproduction in habitats colonized via long-distance di...
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Article
Open AccessComparative transcriptomics reveals commonalities and differences in the genetic underpinnings of a floral dimorphism
Distyly, a floral dimorphism associated with heteromorphic self-incompatibility and controlled by the S-locus supergene, evolved independently multiple times. Comparative analyses of the first transcriptome atlas...
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Protocol
Purification and Reconstitution of the S. cerevisiae TRAMP and Ski Complexes for Biochemical and Structural Studies
The RNA exosome is a macromolecular machine that degrades a large variety of RNAs from their 3′-end. It comprises the major 3′-to-5′ exonuclease in the cell, completely degrades erroneous and overly abundant R...
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Article
InsP6 binding to PIKK kinases revealed by the cryo-EM structure of an SMG1–SMG8–SMG9 complex
We report the 3.45-Å resolution cryo-EM structure of human SMG1–SMG8–SMG9, a phosphatidylinositol-3-kinase (PI(3)K)-related protein kinase (PIKK) complex central to messenger RNA surveillance. Structural and M...
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Article
Open AccessThe MTR4 helicase recruits nuclear adaptors of the human RNA exosome using distinct arch-interacting motifs
The nuclear exosome and its essential co-factor, the RNA helicase MTR4, play crucial roles in several RNA degradation pathways. Besides unwinding RNA substrates for exosome-mediated degradation, MTR4 associate...
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Article
Open AccessDas RNA-Exosom – eine molekulare Maschine für den RNA-Abbau
The exosome is an evolutionary conserved macromolecular complex that degrades RNAs from the 3′ end. It is present both in the cytoplasm and the nucleus, where it degrades different types of RNAs in yeast, huma...
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Article
Open AccessReconstitution of the complete pathway of ITS2 processing at the pre-ribosome
Removal of internal transcribed spacer 2 (ITS2) from pre-ribosomal RNA is essential to make functional ribosomes. This complicated processing reaction begins with a single endonucleolytic cleavage followed by ...
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Article
Open AccessFossils matter: improved estimates of divergence times in Pinus reveal older diversification
The taxonomy of pines (genus Pinus) is widely accepted and a robust gene tree based on entire plastome sequences exists. However, there is a large discrepancy in estimated divergence times of major pine clades am...
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Article
Surviving in isolation: genetic variation, bottlenecks and reproductive strategies in the Canarian endemic Limonium macrophyllum (Plumbaginaceae)
Oceanic archipelagos are typically rich in endemic taxa, because they offer ideal conditions for diversification and speciation in isolation. One of the most remarkable evolutionary radiations on the Canary Is...
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Article
Open AccessStructure of the RBM7–ZCCHC8 core of the NEXT complex reveals connections to splicing factors
The eukaryotic RNA exosome participates extensively in RNA processing and degradation. In human cells, three accessory factors (RBM7, ZCCHC8 and hMTR4) interact to form the nuclear exosome targeting (NEXT) com...
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Article
Reproductive biology of an Alpic paleo-endemic in a changing climate
Climate change is known to have a profound influence on plant reproduction, mainly because it affects plant/pollinator interactions, sometimes driving plants to extinction. Starting from the Neogene, the Euro...
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Article
Knowing the past to forecast the future: a case study on a relictual, endemic species of the SW Alps, Berardia subacaulis
Future climate change may lead to a substantial loss of biodiversity, particularly affecting mountain regions, including the Alps. Range-size reduction in high mountain plant species is predicted to be more pr...
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Article
High genetic diversity and population structure in the endangered Canarian endemic Ruta oreojasme (Rutaceae)
Insular species are expected to have low genetic diversity, for their populations are often small and isolated, and characterized by restricted gene flow and increased incidence of inbreeding. However, empiric...
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Article
Open AccessPhylogeny and biogeography of Primula sect. Armerina: implications for plant evolution under climate change and the uplift of the Qinghai-Tibet Plateau
The historical orogenesis and associated climatic changes of mountain areas have been suggested to partly account for the occurrence of high levels of biodiversity and endemism. However, their effects on dispe...
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Article
RNA degradation paths in a 12-subunit nuclear exosome complex
The eukaryotic exosome is a conserved RNA-degrading complex that functions in RNA surveillance, turnover and processing. How the same machinery can either completely degrade or precisely trim RNA substrates ha...
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Article
Open AccessThe draft genome of Primula veris yields insights into the molecular basis of heterostyly
The flowering plant Primula veris is a common spring blooming perennial that is widely cultivated throughout Europe. This species is an established model system in the study of the genetics, evolution, and ecolog...
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Article
The structure of the Pan2–Pan3 core complex reveals cross-talk between deadenylase and pseudokinase
Conti and colleagues present the crystal structure of the yeast deadenylase Pan2–Pan3 core complex, revealing a 1:2 stoichiometry and indicating how deadenylase and pseudokinase domains work together to promot...
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Article
Biodiversity only makes sense in the light of evolution
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Article
Low genetic diversity and high levels of inbreeding in the Sinai primrose (Primula boveana), a species on the brink of extinction
The Sinai primrose (Primula boveana) is one of the most endangered plant species worldwide, with less than 200 wild individuals surviving in the Sinai mountains of Egypt. There has been a decline in both the numb...
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Article
Structure and RNA-binding properties of the Not1–Not2–Not5 module of the yeast Ccr4–Not complex
The Ccr4–Not complex is involved in several aspects of gene expression, including mRNA decay, translational repression and transcription. Structural, biochemical and functional analyses of the Not module, comp...
