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Article
Towards large scale methods for the selective release of periplasmic human cystatin C from E. coli
Two promising methods, osmotic shock and guanidine treatment, for the selective release of human cystatin C from the periplasmic space of E. coli were developed at the laboratory scale. High release efficiencies ...
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Article
Haemolysis, a tool to evaluate mechanical stress to cells in bioprocess equipment
Suspensions of fragile sheep erythrocytes were used to compare the levels of mechanical stress in two different crossflow filter units of small pilot scale. The recycling pumps were identified as the critical ...
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Article
Procedure for concentration and conditioning of hybridoma cell culture medium for monoclonal antibody purification
Concentration and conditioning the hybridoma cell culture medium is an important step in the monoclonal antibody purification procedure. This report describes a frequent first-step concentration of the hybrido...
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Article
Separation of colour compounds from lipase in fermentation supernatant by diafiltration
Extracellular lipase was produced by growing Geotrichum candidum. A simple optical method was developed to quantify the dark-brown compounds formed during medium preparation and fermentation. The size of these mo...