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    Article

    Towards large scale methods for the selective release of periplasmic human cystatin C from E. coli

    Two promising methods, osmotic shock and guanidine treatment, for the selective release of human cystatin C from the periplasmic space of E. coli were developed at the laboratory scale. High release efficiencies ...

    F. Chaib, A. Bernier, E. Braendli in Biotechnology Techniques (1995)

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    Article

    Haemolysis, a tool to evaluate mechanical stress to cells in bioprocess equipment

    Suspensions of fragile sheep erythrocytes were used to compare the levels of mechanical stress in two different crossflow filter units of small pilot scale. The recycling pumps were identified as the critical ...

    M. Oliver, E. Braendli in Biotechnology Techniques (1991)

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    Article

    Procedure for concentration and conditioning of hybridoma cell culture medium for monoclonal antibody purification

    Concentration and conditioning the hybridoma cell culture medium is an important step in the monoclonal antibody purification procedure. This report describes a frequent first-step concentration of the hybrido...

    K. Veeraragavan, A. Bernier, M. Oliver, E. Braendli in Journal of tissue culture methods (1991)

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    Article

    Separation of colour compounds from lipase in fermentation supernatant by diafiltration

    Extracellular lipase was produced by growing Geotrichum candidum. A simple optical method was developed to quantify the dark-brown compounds formed during medium preparation and fermentation. The size of these mo...

    M. Pacheco-Oliver, K. Veeraragavan, E. Braendli in Biotechnology Techniques (1990)