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Procedure for concentration and conditioning of hybridoma cell culture medium for monoclonal antibody purification

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Journal of tissue culture methods

Summary

Concentration and conditioning the hybridoma cell culture medium is an important step in the monoclonal antibody purification procedure. This report describes a frequent first-step concentration of the hybridoma cell culture medium and the conditioning of the concentrated medium for the affinity purification of the monoclonal antibodies.

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Authors and Affiliations

  1. BioSeparation Group, Biochemical Engineering Section, Biotechnology Research Institute, National Research Council of Canada, H4P 2R2, Montreal, Canada

    K. Veeraragavan, A. Bernier, M. Oliver & E. Braendli

Authors
  1. K. Veeraragavan
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  2. A. Bernier
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  3. M. Oliver
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  4. E. Braendli
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Veeraragavan, K., Bernier, A., Oliver, M. et al. Procedure for concentration and conditioning of hybridoma cell culture medium for monoclonal antibody purification. Journal of Tissue Culture Methods 13, 49–51 (1991). https://doi.org/10.1007/BF02388204

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  • DOI: https://doi.org/10.1007/BF02388204

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