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Article
The kinetic landscape of an RNA-binding protein in cells
Gene expression in higher eukaryotic cells orchestrates interactions between thousands of RNA-binding proteins (RBPs) and tens of thousands of RNAs1. The kinetics by which RBPs bind to and dissociate from their R...
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Article
Map** transcriptome-wide protein-RNA interactions to elucidate RNA regulatory programs
Our understanding of post-transcriptional gene regulation has increased exponentially with the development of robust methods to define protein-RNA interactions across the transcriptome. In this review, we high...
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Article
The helicase Ded1p controls use of near-cognate translation initiation codons in 5′ UTRs
The conserved and essential DEAD-box RNA helicase Ded1p from yeast and its mammalian orthologue DDX3 are critical for the initiation of translation1. Mutations in DDX3 are linked to tumorigenesis2–4 and intellect...
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Article
IL-17-receptor-associated adaptor Act1 directly stabilizes mRNAs to mediate IL-17 inflammatory signaling
Mechanisms that degrade inflammatory mRNAs are well known; however, stabilizing mechanisms are poorly understood. Here, we show that Act1, an interleukin-17 (IL-17)-receptor-complex adaptor, binds and stabiliz...
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Chapter
Roles of RNA-binding Proteins and Post-transcriptional Regulation in Driving Male Germ Cell Development in the Mouse
Tissue development and homeostasis are dependent on highly regulated gene expression programs in which cell-specific combinations of regulatory factors determine which genes are expressed and the post-transcri...
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Protocol
3′ End Formation and Regulation of Eukaryotic mRNAs
The polyadenosine (polyA) “tail” is an essential feature at the 3′ end of nearly all eukaryotic mRNAs. This appendage has roles in many steps in the gene expression pathway and is subject to extensive regulati...
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Article
RNA processing and its regulation: global insights into biological networks
The limited differences between the genomes of very different species have led to the emerging recognition that biological diversity is likely to largely deriv...
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Article
HITS-CLIP yields genome-wide insights into brain alternative RNA processing
Protein–RNA interactions have critical roles in all aspects of gene expression. However, applying biochemical methods to understand such interactions in living tissues has been challenging. Here we develop a g...
