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Article
Open AccessAuthor Correction: Guidelines and definitions for research on epithelial–mesenchymal transition
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Article
Open AccessGuidelines and definitions for research on epithelial–mesenchymal transition
Epithelial–mesenchymal transition (EMT) encompasses dynamic changes in cellular organization from epithelial to mesenchymal phenotypes, which leads to functional changes in cell migration and invasion. EMT occ...
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Protocol
Technologies for Live Imaging of Enteric Neural Crest-Derived Cells
Time-lapse imaging of gut explants from embryonic mice in which neural crest-derived cells express fluorescent proteins allows the behavior of enteric neural crest cells to be observed and analyzed. Explants o...
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Protocol
Generation of Neural Crest Progenitors from Human Pluripotent Stem Cells
There are a vast range of diseases and disorders that are neurocristopathic in origin, including Hirschsprung’s disease, pheochromocytoma, familial dysautonomia, craniofacial disorders, and melanomas. Having a...
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Protocol
Addressing Interdisciplinary Difficulties in Developmental Biology/Mathematical Collaborations: A Neural Crest Example
Mathematical modeling can allow insight into the biological processes that can be difficult to access by conventional biological means alone. Such projects are becoming increasingly attractive with the appeara...
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Chapter
PCA Modelling of Multi-species Cell Clusters: Ganglion Development in the Gastrointestinal Nervous System
A defining characteristic of the enteric nervous system (ENS) is mesoscale patterned entities called ganglia. Ganglia are clusters of neurons with associated enteric neural crest (ENC) cells, which form in the...
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Article
Open AccessCRISPR/Cas9 Targets Chicken Embryonic Somatic Cells In Vitro and In Vivo and generates Phenotypic Abnormalities
Chickens are an invaluable model for studying human diseases, physiology and especially development, but have lagged in genetic applications. With the advent of Programmable Engineered Nucleases, genetic manip...
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Article
Open AccessDirect repression of MYB by ZEB1 suppresses proliferation and epithelial gene expression during epithelial-to-mesenchymal transition of breast cancer cells
Epithelial-to-mesenchymal transition (EMT) promotes cell migration and is important in metastasis. Cellular proliferation is often downregulated during EMT, and the reverse transition (MET) in metastases appea...
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Article
Trans-mesenteric neural crest cells are the principal source of the colonic enteric nervous system
In this study, the authors show that, during the development of the enteric nervous system, a subset of enteric neural crest cells migrate from midgut across the mesentery to colonize caudal hindgut. This tran...
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Article
Contribution of Fibroblast and Mast Cell (Afferent) and Tumor (Efferent) IL-6 Effects within the Tumor Microenvironment
Hyperactive inflammatory responses following cancer initiation have led to cancer being described as a ‘wound that never heals’. These inflammatory responses elicit signals via NFκB leading to IL-6 production,...
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Article
Spatial Analysis of Multi-species Exclusion Processes: Application to Neural Crest Cell Migration in the Embryonic Gut
Hindbrain (vagal) neural crest cells become relatively uniformly distributed along the embryonic intestine during the rostral to caudal colonization wave which forms the enteric nervous system (ENS). When vaga...
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Chapter and Conference Paper
Modeling Development and Disease in Our “Second” Brain
The enteric nervous system (ENS) in our gastrointestinal tract, nicknamed the “second brain”, is responsible for normal gut function and peristaltic contraction. Embryonic development of the ENS involves the c...
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Article
Open AccessStaurosporine augments EGF-mediated EMT in PMC42-LA cells through actin depolymerisation, focal contact size reduction and Snail1 induction – A model for cross-modulation
A feature of epithelial to mesenchymal transition (EMT) relevant to tumour dissemination is the reorganization of actin cytoskeleton/focal contacts, influencing cellular ECM adherence and motility. This is cou...
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Protocol
Analysing Tissue and Gene Function in Intestinal Organ Culture
The study of growth, differentiation, and migration of different cell types within the develo** intestine has been enhanced by the development of methods to grow intestinal tissue in organ culture. Here, we ...
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Article
Open AccessInduction of epithelial to mesenchymal transition in PMC42-LA human breast carcinoma cells by carcinoma-associated fibroblast secreted factors
Breast carcinoma is accompanied by changes in the acellular and cellular components of the microenvironment, the latter typified by a switch from fibroblasts to myofibroblasts.
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Article
Myoepithelial molecular markers in human breast carcinoma PMC42-LA cells are induced by extracellular matrix and stromal cells
The microenvironment plays a key role in the cellular differentiation of the two main cell lineages of the human breast, luminal epithelial, and myoepithelial. It is not clear, however, how the components of t...
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Chapter
Neural Crest and the Development of the Enteric Nervous System
The formation of the enteric nervous system (ENS) is a particularly interesting example of the migratory ability of the neural crest and of the complexity of structures to which neural crest cells contribute. ...
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Chapter
The Neural Crest
The concept of the epithelial-mesenchymal transition (EMT) arose from developmental biology, (see ref. 1) where EMT occurs in many situations, each being predictable, stereotyped and with the outcomes often dr...
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Chapter
Matrix Metalloproteases and Epithelial-to-Mesenchymal Transition
The epithelial to mesenchymal transition (EMT) is characterized by the loss of epithelial characteristics and the gain of mesenchymal attributes in epithelial cells. It has been associated with physiological a...
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Protocol
Neural Crest Cell Outgrowth Cultures and the Analysis of Cell Migration
The neural crest gives rise to many differentiated cell types including craniofacial connective tissue, peripheral neurons, Schwann cells, various endocrine organs, and pigment cells and it has been extensivel...
