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Protocol
Neural Crest Cell Outgrowth Cultures and the Analysis of Cell Migration
The neural crest gives rise to many differentiated cell types including craniofacial connective tissue, peripheral neurons, Schwann cells, various endocrine organs, and pigment cells and it has been extensivel...
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Chapter
The Neural Crest
The concept of the epithelial-mesenchymal transition (EMT) arose from developmental biology, (see ref. 1) where EMT occurs in many situations, each being predictable, stereotyped and with the outcomes often dr...
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Chapter
Matrix Metalloproteases and Epithelial-to-Mesenchymal Transition
The epithelial to mesenchymal transition (EMT) is characterized by the loss of epithelial characteristics and the gain of mesenchymal attributes in epithelial cells. It has been associated with physiological a...
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Chapter
Neural Crest and the Development of the Enteric Nervous System
The formation of the enteric nervous system (ENS) is a particularly interesting example of the migratory ability of the neural crest and of the complexity of structures to which neural crest cells contribute. ...
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Article
Myoepithelial molecular markers in human breast carcinoma PMC42-LA cells are induced by extracellular matrix and stromal cells
The microenvironment plays a key role in the cellular differentiation of the two main cell lineages of the human breast, luminal epithelial, and myoepithelial. It is not clear, however, how the components of t...
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Protocol
Technologies for Live Imaging of Enteric Neural Crest-Derived Cells
Time-lapse imaging of gut explants from embryonic mice in which neural crest-derived cells express fluorescent proteins allows the behavior of enteric neural crest cells to be observed and analyzed. Explants o...
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Protocol
Generation of Neural Crest Progenitors from Human Pluripotent Stem Cells
There are a vast range of diseases and disorders that are neurocristopathic in origin, including Hirschsprung’s disease, pheochromocytoma, familial dysautonomia, craniofacial disorders, and melanomas. Having a...
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Protocol
Addressing Interdisciplinary Difficulties in Developmental Biology/Mathematical Collaborations: A Neural Crest Example
Mathematical modeling can allow insight into the biological processes that can be difficult to access by conventional biological means alone. Such projects are becoming increasingly attractive with the appeara...
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Article
Open AccessGuidelines and definitions for research on epithelial–mesenchymal transition
Epithelial–mesenchymal transition (EMT) encompasses dynamic changes in cellular organization from epithelial to mesenchymal phenotypes, which leads to functional changes in cell migration and invasion. EMT occ...
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Article
Open AccessAuthor Correction: Guidelines and definitions for research on epithelial–mesenchymal transition