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Article
Open AccessTargeted depletion of TRBV9+ T cells as immunotherapy in a patient with ankylosing spondylitis
Autoimmunity is intrinsically driven by memory T and B cell clones inappropriately targeted at self-antigens. Selective depletion or suppression of self-reactive T cells remains a holy grail of autoimmune ther...
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Article
An overview of immunoinformatics approaches and databases linking T cell receptor repertoires to their antigen specificity
Recent advances in molecular and bioinformatic methods have greatly improved our ability to study the formation of an adaptive immune response towards foreign pathogens, self-antigens, and cancer neoantigens. ...
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Article
Open AccessExploring the pre-immune landscape of antigen-specific T cells
Adaptive immune responses to newly encountered pathogens depend on the mobilization of antigen-specific clonotypes from a vastly diverse pool of naive T cells. Using recent advances in immune repertoire sequen...
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Article
Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy
Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue...
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Article
Local fitness landscape of the green fluorescent protein
Comprehensive genotype–phenotype map** of the green fluorescent protein shows that the local fitness peak is narrow, shaped by a high prevalence of epistatic interactions, providing for the loss of fluoresce...
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Article
Open AccesstcR: an R package for T cell receptor repertoire advanced data analysis
The Immunoglobulins (IG) and the T cell receptors (TR) play the key role in antigen recognition during the adaptive immune response. Recent progress in next-generation sequencing technologies has provided an o...
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Article
Open AccessOptogenetic in vivocell manipulation in KillerRed-expressing zebrafish transgenics
KillerRed (KR) is a novel photosensitizer that efficiently generates reactive oxygen species (ROS) in KR-expressing cells upon intense green or white light illumination in vitro, resulting in damage to their plas...
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Article
Photoactivatable fluorescent proteins
The fluorescence characteristics of photoactivatable proteins can be controlled by irradiating them with light of a specific wavelength, intensity and duration. This provides unique possibilities for the optic...
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Article
Open AccessInterconversion of Anthozoa GFP-like fluorescent and non-fluorescent proteins by mutagenesis
Within the family of green fluorescent protein (GFP) homologs, one can mark two main groups, specifically, fluorescent proteins (FPs) and non-fluorescent or chromoproteins (CPs). Structural background of diffe...
