-
Protocol
Dielectrophoresis-Assisted Self-Digitization Chip for High-Efficiency Single-Cell Analysis
Single-cell analysis of cell phenotypic information such as surface protein expression and nucleic acid content is essential for understanding heterogeneity within cell populations. Here the design and use of ...
-
Protocol
Digital Quantification of Human Viral RNA and DNA Using a Self-Digitization Chip
Digital nucleic acid quantitation methods show excellent sensitivity and specificity for pathogen detection. Droplet digital PCR (ddPCR) is the most advanced digital nucleic acid quantitation method and has be...
-
Article
Open AccessOptical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots
The efficient selection and isolation of individual cells of interest from a mixed population is desired in many biomedical and clinical applications. Here we show the concept of using photoswitchable semicond...
-
Protocol
Ultrasensitive Protein Detection on Dot Blots and Western Blots with Semiconducting Polymer Dots
We demonstrate ultrasensitive fluorescence imaging of proteins on dot blots and western blots using a bright, compact, and orange-emitting semiconducting polymer dot (CNPPV). We achieved a detection limit at t...
-
Protocol
Single-Cell Nanosurgery
This chapter explains the steps necessary to perform laser surgery upon single adherent mammalian cells, where individual organelles are extracted from the cells by optical tweezers and the cells are monitored...
-
Article
Determining the number of specific proteins in cellular compartments by quantitative microscopy
This protocol describes a method for determining both the average number and variance of proteins, in the few to tens of copies, in isolated cellular compartments such as organelles and protein complexes. Othe...
-
Article
Interfacing droplet microfluidics with chemical separation for cellular analysis
This trends article discusses the interface between droplet microfluidics and micro-scale chemical separation. Droplet microfluidics has witnessed explosive growth over the past few years, but the use of dropl...
-
Article
Cellular manipulations in microvortices
-
Article
High radial acceleration in microvortices
Microfluidic systems can conveniently be used for rapid analysis of biological samples1,2,3,4,5. Here we describe a single re-circulating flow, or microvortex, that can generate a maximum fluid rotational velocit...
-
Article
Microfluidics Section: Design and Fabrication of Integrated Passive Valves and Pumps for Flexible Polymer 3-Dimensional Microfluidic Systems
This paper describes the fabrication of flexible, polymeric 3-dimensional microfluidic systems with integrated check valves (flap and diaphragm valves) and a pump by stacking patterned poly(dimethylsiloxane) (...
