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Article
POSITIVELY CHARGED OLIGONUCLEOTIDES OVERCOME POTASSIUM-MEDIATED INHIBITION OF TRIPLEX DNA FORMATION. † 847
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Article
Positively Charged Oligonucleotides as Regulators of Gene Expression in Vivo• 240
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Article
Changes in Heart Development in Response to Alteration of Nkx2.3 and Nkx2.5 in Xenopus Embryos
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Article
Transgenic Xenopus laevis embryos can be generated using φC31 integrase
Bacteriophage φC31 encodes an integrase that can mediate the insertion of extrachromosomal DNA into genomic DNA. Here we show that the coinjection of mRNA encoding φC31 integrase with plasmid DNA encoding the ...
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Article
Using phiC31 integrase to make transgenic Xenopus laevis embryos
Bacteriophage phiC31 produces the enzyme integrase that allows the insertion of the phage genome into its bacterial host. This enzyme recognizes a specific DNA sequence in the phage (attP) and a different sequenc...
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Protocol
Bacteriophage φC31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels
Bacteriophage φC31 inserts its genome into that of its host bacterium via the integrase enzyme which catalyzes recombination between a phage attachment site (attP) and a bacterial attachment site (attB). Integras...
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Article
Examining the Cardiac NK-2 Genes in Early Heart Development
The cardiac NK-2 transcription factors are the vertebrate relatives of the Drosophila tinman gene. Without the Drosophila tinman gene, fruit flies fail to form their heart (“dorsal vessel”), and mutations or alte...
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Protocol
Using ΦC31 Integrase to Mediate Insertion of DNA in Xenopus Embryos
The two most common methods used to generate transgenic Xenopus embryos, restriction enzyme-mediated insertion, and I-SceI meganuclease take advantage of relatively common but spatially unpredictable double-stran...