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  1. No Access

    Protocol

    Using ΦC31 Integrase to Mediate Insertion of DNA in Xenopus Embryos

    The two most common methods used to generate transgenic Xenopus embryos, restriction enzyme-mediated insertion, and I-SceI meganuclease take advantage of relatively common but spatially unpredictable double-stran...

    You E. Li, Bryan G. Allen, Daniel L. Weeks in Xenopus Protocols (2012)

  2. No Access

    Article

    Examining the Cardiac NK-2 Genes in Early Heart Development

    The cardiac NK-2 transcription factors are the vertebrate relatives of the Drosophila tinman gene. Without the Drosophila tinman gene, fruit flies fail to form their heart (“dorsal vessel”), and mutations or alte...

    Heather Bartlett, Gert Jan C. Veenstra, Daniel L. Weeks in Pediatric Cardiology (2010)

  3. No Access

    Protocol

    Bacteriophage φC31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels

    Bacteriophage φC31 inserts its genome into that of its host bacterium via the integrase enzyme which catalyzes recombination between a phage attachment site (attP) and a bacterial attachment site (attB). Integras...

    Bryan G. Allen, Daniel L. Weeks in Microinjection (2009)

  4. No Access

    Article

    Using phiC31 integrase to make transgenic Xenopus laevis embryos

    Bacteriophage phiC31 produces the enzyme integrase that allows the insertion of the phage genome into its bacterial host. This enzyme recognizes a specific DNA sequence in the phage (attP) and a different sequenc...

    Bryan G Allen, Daniel L Weeks in Nature Protocols (2006)

  5. No Access

    Article

    Transgenic Xenopus laevis embryos can be generated using φC31 integrase

    Bacteriophage φC31 encodes an integrase that can mediate the insertion of extrachromosomal DNA into genomic DNA. Here we show that the coinjection of mRNA encoding φC31 integrase with plasmid DNA encoding the ...

    Bryan G Allen, Daniel L Weeks in Nature Methods (2005)

  6. Article

    Changes in Heart Development in Response to Alteration of Nkx2.3 and Nkx2.5 in Xenopus Embryos

    Urszula W Tajchman, Sandra J Kolker, Vera M Ogniewski, John M Dagle… in Pediatric Research (1999)

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  7. Article

    Positively Charged Oligonucleotides as Regulators of Gene Expression in Vivo• 240

    John M. Dagle, Daniel L. Weeks in Pediatric Research (1997)

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  8. Article

    POSITIVELY CHARGED OLIGONUCLEOTIDES OVERCOME POTASSIUM-MEDIATED INHIBITION OF TRIPLEX DNA FORMATION. † 847

    John M Dagle, Daniel L Weeks in Pediatric Research (1996)

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