19 Result(s)
within
Christopher T. Nomura
-
Article
Engineering the Tat-secretion pathway of Bacillus licheniformis for the secretion of cytoplasmic enzyme arginase
The industrial bacterium Bacillus licheniformis has long been used as a microbial factory for the production of enzymes due to its ability to secrete copious amounts of native extracellular proteins and its gener...
-
Article
Production of polyhydroxybutyrate and polyhydroxybutyrate-co-MCL copolymers from brewer’s spent grains by recombinant Escherichia coli LSBJ
This study investigates the use of brewer’s spent grains (BSG), a major waste stream from the brewing process, for its potential as a carbon source for the fermentative production of polyhydroxyalkanoates (PHA...
-
Article
Open AccessSuperior thermal stability and fast crystallization behavior of a novel, biodegradable α-methylated bacterial polyester
Given their ubiquity in modern society, the development of biodegradable and renewably sourced plastics is essential for the creation of an environmentally sustainable society. One of the drawbacks for current...
-
Article
Open AccessUtilization of L-glutamate as a preferred or sole nutrient in Pseudomonas aeruginosa PAO1 depends on genes encoding for the enhancer-binding protein AauR, the sigma factor RpoN and the transporter complex AatJQMP
Glutamate and aspartate are preferred nutrients for a variety of microorganisms. In the case for many Pseudomonas spp., utilization of these amino acids is believed to be dependent on a transporter complex compri...
-
Article
Systematic metabolic pathway modification to boost l-ornithine supply for bacitracin production in Bacillus licheniformis DW2
Bacitracin is a cyclic dodecyl peptide antibiotic that is an effective bacteriocide against Gram-positive and some Gram-negative bacteria. Bacitracin has been widely used as an antibacterial feed additive for ...
-
Article
Increased synthesis of poly(3-hydroxydodecanoate) by random mutagenesis of polyhydroxyalkanoate synthase
Poly(3-hydroxydodecanoate) [P(3HDD)], a medium-chain-length polyhydroxyalkanoate (PHA), is expected to be used as a novel type of bioplastic characterized by a soft and transparent nature. In this study, to ac...
-
Article
Increased flux through the TCA cycle enhances bacitracin production by Bacillus licheniformis DW2
The dodecapeptide antibiotic bacitracin, produced by several strains of Bacillus licheniformis and Bacillus subtilis, is widely used as an antibacterial animal feed additive. Several genetic strategies were explo...
-
Article
Acetolactate synthase (AlsS) in Bacillus licheniformis WX-02: enzymatic properties and efficient functions for acetoin/butanediol and l-valine biosynthesis
Acetolactate synthase catalyzes two molecules of pyruvates to form α-acetolactate, which is further converted to acetoin and 2,3-butanediol. In this study, by heterologous expression in Escherichia coli, the enzy...
-
Article
Optimization of Inexpensive Agricultural By-Products as Raw Materials for Bacitracin Production in Bacillus licheniformis DW2
Bacitracin is a broad-spectrum antibiotic used extensively as a feed additive. In this study, inexpensive agricultural by-products were used as nitrogen sources for bacitracin production. Based on both the ort...
-
Article
Open AccessTargeting the alternative sigma factor RpoN to combat virulence in Pseudomonas aeruginosa
Pseudomonas aeruginosa is a Gram-negative, opportunistic pathogen that infects immunocompromised and cystic fibrosis patients. Treatment is difficult due to antibiotic resistance, and new...
-
Article
Open AccessA novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis
Signal peptide peptidases play an important role in the removal of remnant signal peptides in the cell membrane, a critical step for extracellular protein production. Although these proteins are likely a centr...
-
Article
Use of Bacillus amyloliquefaciens HZ-12 for High-Level Production of the Blood Glucose Lowering Compound, 1-Deoxynojirimycin (DNJ), and Nutraceutical Enriched Soybeans via Fermentation
1-Deoxynojirimycin (DNJ) is an efficient α-glucosidase inhibitor (α-GI) with potential applications in the prevention and treatment of diabetes. In this study, 16 Bacillus strains were screened for α-GI rate, and...
-
Article
Open AccessEnhancing poly(3-hydroxyalkanoate) production in Escherichia coli by the removal of the regulatory gene arcA
Recombinant Escherichia coli is a desirable platform for the production of many biological compounds including poly(3-hydroxyalkanoates), a class of naturally occurring biodegradable polyesters with promising bio...
-
Article
Open AccessEngineering Bacillus licheniformis for the production of meso-2,3-butanediol
2,3-Butanediol (2,3-BD) can be used as a liquid fuel additive to replace petroleum oil, and as an important platform chemical in the pharmaceutical and plastic industries. Microbial production of 2,3-BD by Bacill...
-
Article
Enhanced production of polyhydroxyalkanoates (PHAs) from beechwood xylan by recombinant Escherichia coli
Microbial conversion of plant biomass to value-added products is an attractive option to address the impacts of petroleum dependency. In this study, a bacterial system was developed that can hydrolyze xylan an...
-
Article
Production of polyhydroxyalkanoates by Burkholderia cepacia ATCC 17759 using a detoxified sugar maple hemicellulosic hydrolysate
Sugar maple hemicellulosic hydrolysate containing 71.9 g/l of xylose was used as an inexpensive feedstock to produce polyhydroxyalkanoates (PHAs) by Burkholderia cepacia ATCC 17759. Several inhibitory compounds p...
-
Article
PHA synthase engineering toward superbiocatalysts for custom-made biopolymers
Poly-3-hydroxyalkanoates [P(3HA)s] are biologically produced polyesters that have attracted much attention as biodegradable polymers that can be produced from biorenewable resources. These polymers have many a...
-
Article
Roles for heme–copper oxidases in extreme high-light and oxidative stress response in the cyanobacterium Synechococcus sp. PCC 7002
The ctaCIDIEI and ctaCIIDIIEII gene clusters that encode heme–copper cytochrome oxidases have been characterized in the marine cyanobacterium Synechococcus sp. PCC 7002 and the inactivation of ctaDI was shown to ...
-
Article
Characterization of two cytochrome oxidase operons in the marine cyanobacterium Synechococcus sp. PCC 7002: Inactivation of ctaDI affects the PS I:PS II ratio
Cyanobacteria have versatile electron transfer pathways and many of the proteins involved are functional in both respiratory and photosynthetic electron transport. Examples of such proteins include the cytochr...
