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Article
Analysis and comparison of cloning methods for the preparation of repetitive polypeptides
Repetitive polypeptides, defined as a protein consisting primarily of tandemly repeated blocks of amino acid sequence, are widely used biomaterials. These repetitive polypeptides can be used in diverse biologi...
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Article
One-pot biotransformation of racemic styrene oxide into (R)-1,2-phenylethandiol by two recombinant microbial epoxide hydrolases
An enantioconvergent biotransformation of racemic styrene oxide by using two recombinant microbial epoxide hydrolases (EHs) in one pot has been investigated to prepare enantiopure vicinal diols. The recombinan...
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Article
Enantioconvergent bioconversion of p-chlorostyrene oxide to (R)-p-chlorophenyl-1,2-ethandiol by the bacterial epoxide hydrolase of Caulobacter crescentus
The enantioselective hydrolysis of eight racemic styrene oxide derivatives has been investigated by using the recombinant cell containing epoxide hydrolase (EH) of Caulobacter crescentus. Some styrene oxide deriv...
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Article
Biodegradation of gaseous styrene by Brevibacillus sp. using a novel agitating biotrickling filter
A novel biofilter with an agitator to control excessive biomass accumulation, the agitating biotrickling filter (ABTF) system, was developed for treatment of gaseous styrene using Brevibacillus sp. as the sole mi...
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Article
Bioconversion of 12-, 14-, and 16-membered ring aglycones to glycosylated macrolides in an engineered strain of Streptomyces venezuelae
To develop a system for combinatorial biosynthesis of glycosylated macrolides, Streptomyces venezuelae was genetically manipulated to be deficient in the production of its macrolide antibiotics by deletion of the...
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Article
Use ofl-buthionine sulfoximine for the efficient expression of disulfide-containing proteins in cell-free extracts ofEscherichia coli
We have developed a technique to improve the formation of correct disulfide bonds within cell-free synthesized proteins. Via the use of a metabolic inhibitor of glutamate-cysteine ligase, the accumulation of g...
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Article
Generation of a naïve/synthetic antibody specific to botulinum neurotoxin via motif-grafting
In this study, we describe a new approach to the production of naïve/synthetic human antibodies against the botulinum neurotoxin (BoNT). First, peptides that bind to BoNT serotype A (BoNT/A) were screened from...
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Article
Additive effect of endothelial progenitor cell mobilization and bone marrow mononuclear cell transplantation on angiogenesis in mouse ischemic limbs
The methods of therapeutic angiogenesis include endothelial progenitor cell (EPC) mobilization with cytokines [e.g., granulocyte colony-stimulating factor (G-CSF)] and bone marrow mononuclear cell (BMMNC) tran...
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Article
Development and mathematical modeling of a two-stage reactor system for trichloroethylene degradation using Methylosinus trichosporium OB3b
A two-stage reactor system was developed for the continuous degradation of gas-phase trichloroethylene (TCE). Methylosinus trichosporium OB3b was immobilized on activated carbon in a TCE degradation reactor, tric...
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Article
Heterologous expression of tylosin polyketide synthase and production of a hybrid bioactive macrolide in Streptomyces venezuelae
Tylosin polyketide synthase (Tyl PKS) was heterologously expressed in an engineered strain of Streptomyces venezuelae bearing a deletion of pikromycin PKS gene cluster using two compatible low-copy plasmids, each...
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Article
Preparation method forEscherichia coli S30 extracts completely dependent upon tRNA addition to catalyze cell-free protein synthesis
A simple method for depletingE. coli S30 extracts of endogenous tRNA has been developed. An ethanolamine-Sepharose® column equilibrated with water selectively captured the tRNA molecules inE. coli S30 extracts. A...
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Article
Development of a rapid and productive cell-free protein synthesis system
Due to recent advances in genome sequencing, there has been a dramatic increase in the quantity of genetic information, which has lead to an even greater demand for a faster, more parallel expression system. T...
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Article
Zeolite-mediated cation exchange enhances the stability of mRNA during cell-free protein synthesis
The addition of zeolite particles enhances the stability of mRNA molecules in a cell-free protein synthesis system. When 20 μg/μL of zeolite (Y5.4) is added to a reaction mixture of cell-free protein synthesis...
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Article
Open AccessTissue engineering of heart valves by recellularization of glutaraldehyde-fixed porcine valves using bone marrow-derived cells
To increase the biocompatibility and durability of glutaraldehyde (GA)-fixed valves, a biological coating with viable endothelial cells (ECs) has been proposed. However, stable EC layers have not been formed s...
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Article
Production of chiral epoxides: Epoxide hydrolase-catalyzed enantioselective hydrolysis
Chiral epoxides are highly valuable intermediates, used for the synthesis of pharmaceutical drugs and agrochemicals. They have broad scope of market demand because of their applications. A major challenge in m...
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Article
Construction of CpG motif-enriched DNA vaccine plasmids for enhanced early immune response
A DNA vaccine methodology using eukaryote expression vectors to produce immunizing proteins in the vaccinated hosts is a novel approach to the development of vaccine and immuno-therapeutics, and it has achieve...
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Article
Minimally complex problem set for anAb Initio protein structure prediction study
A “minimally complex problem set” forab initio protein structure prediction has been proposed. As well as consisting of non-redundant and crystallographically determined high-resolution protein structures, withou...
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Article
Enhanced immunodetection of cell-free synthesized erythropoietin under denaturing conditions
A monoclonal antibody produced by hydridoma cell line, ATCC HB8209, was used to detect and purify erythropoietin synthesized in a cell-free system. The antibody was raised against the N-terminal 20 residues of er...
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Article
Renal tissue reconstitution by the implantation of renal segments on biodegradable polymer scaffolds
Renal units were created in vivo by transplanting isolated renal segments on three-dimensional, biodegradable polymer scaffolds. Renal segments, freshly isolated from rat kidneys, were seeded on polymer scaffolds...
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Article
Suspension culture of anchorage-dependent animal cells using nanospheres of the biodegradable polymer, poly(lactic-co-glycolic acid)
A novel method was developed for suspension culture of anchorage-dependent animal cells using biodegradable polymer nanospheres. The addition of poly(lactic-co-glycolic acid) nanospheres (907 nm average diam.)...
