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Article
Open AccessModeling cellular responses to serum and vitamin D in microgravity using a human kidney microphysiological system
The microgravity environment aboard the International Space Station (ISS) provides a unique stressor that can help understand underlying cellular and molecular drivers of pathological changes observed in astro...
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Article
Open AccessDevelopment of a kidney microphysiological system hardware platform for microgravity studies
Determining the physiological effects of microgravity on the human kidney is limited to relatively insensitive tests of biofluids (blood and urine) that do not return abnormal results until more than 50% of ki...
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Article
Incorporating Uremic Solute-mediated Inhibition of OAT1/3 Improves PBPK Prediction of Tenofovir Renal and Systemic Disposition in Patients with Severe Kidney Disease
Dose modification of renally secreted drugs in patients with chronic kidney disease (CKD) has relied on serum creatinine concentration as a biomarker to estimate glomerular filtration (GFR) under the assumptio...
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Article
Open AccessBridging the gap between in silico and in vivo by modeling opioid disposition in a kidney proximal tubule microphysiological system
Opioid overdose, dependence, and addiction are a major public health crisis. Patients with chronic kidney disease (CKD) are at high risk of opioid overdose, therefore novel methods that provide accurate predic...
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Article
Open AccessCoenzyme Q10 dose-escalation study in hemodialysis patients: safety, tolerability, and effect on oxidative stress
Coenzyme Q10 (CoQ10) supplementation improves mitochondrial coupling of respiration to oxidative phosphorylation, decreases superoxide production in endothelial cells, and may improve functional cardiac capacity ...
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Article
e-PKGene: A knowledge-based research tool for analysing the impact of genetics on drug exposure
e-PKGene (http://www.pharmacogeneticsinfo.org) is a manually curated knowledge product developed in the Department of Pharmaceutics at the University of Wash...
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Chapter
Drug Disposition and Drug–Drug Interactions: Importance of First-Pass Metabolism in Gut and Liver
Drugs with high intestinal and hepatic extraction that undergo biotransformation are often involved in significant metabolic drug–drug interactions when co-administered with enzyme inhibitors or inducers. Thes...
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Protocol
Benzydamine N-Oxygenation as a Measure of Flavin-Containing Monooxygenase Activity
Benzydamine is a nonsteroidal anti-inflammatory drug that undergoes flavin-containing monooxygenase (FMO)-dependent metabolism to a stable N-oxide. This metabolite can be quantified with high specificity and sens...
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Protocol
Prochiral Sulfoxidation as a Probe for Flavin-Containing Monooxygenases
Asymmetric aryl alkyl sulfides (R-S-R′) are metabolized by flavin-containing monooxygenase (FMO) and cytochrome P450 enzymes to enantiomerically enriched sulfoxide products (R-SO-R′) that are readily analyzed ...
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Article
Human Hepatic Flavin-Containing Monooxygenases 1 (FMO1) and 3 (FMO3) Developmental Expression
The flavin-containing monooxygenases (FMOs) are important for the metabolism of numerous therapeutics and toxicants. Six mammalian FMO genes (FMO1–6) have been identified, each exhibiting developmental and tissue...
