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Article
Expression of an antibody-targeted plasminogen activator in Escherichia coli using chemically induced stress responses
A recombinant gene coding for an antibody-targeted urokinase-type plasminogen activator was constructed for the purpose of enhancing the thrombolytic specificity of urokinase. The recombinant gene was cloned i...
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Article
Enhanced expression of an antibody-targeted plasminogen activator in Escherichia coli by fusion to decorsin
To improve the thrombolytic specificity of plasminogen activators, an antibody-targeted plasminogen activator was constructed consisting of a single-chain variable fragment of a monoclonal antibody SZ-51 raise...
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Chapter and Conference Paper
Earthworms: Sources of Antimicrobial and Anticancer Molecules
According to Reynolds and Reynolds, (1972) few people know of the earthworm’s long association with medicine, yet documents recording its use in various remedies date back to 1340 A.D.1, 2 For instance, doctors p...
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Article
Rheb is a direct target of the tuberous sclerosis tumour suppressor proteins
Mutations in the TSC1 or TSC2 genes cause tuberous sclerosis, a benign tumour syndrome in humans1,2. Tsc2 possesses a domain that shares homology with the GTPase-activating protein (GAP) domain of Rap1-GAP2, sugg...
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Article
Characteristics of mixotrophic growth of Synechocystis sp. in an enclosed photobioreactor
Synechocystis sp. PCC 6803 was grown in a 2.5 l enclosed photobioreactor on medium with or without glucose. The incident light intensities ranged from 1.5 klux to 7 klux. The highest average specific growth rate...
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Article
Comparative Proteome Analysis of Human Temporal Cortex Lobes by Two-Dimensional Electrophoresis and Identification of Selected Common Proteins
Human brain proteins were isolated from left and right temporal cortex lobes at the age of 73, 23, 84 years and separated by two-dimensional gel electrophoresis (2-DE). 2-DE was carried out with an immobilized...
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Article
Neuronal growth inhibitory factor inhibits pheochromocytoma PC 12 in vitro
Neuronal growth inhibitory factor (GIF), named Metallothioneins-III (MT-III), is the first protein validated to be capable of inhibiting the growth of neurons in nervous system. We have detected the effects of...
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Article
A prourokinase-RGDS chimera
A tetrapeptide, RGDS, was inserted into proUK kringle domain G118-L119 by the construction of a mutant proUK-RGDS gene. The gene was expressed in the baculovirus expression system. Immunoaffinity chromatograph...
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Chapter
Molecular splicing of metallothionein — study on domains of metallothionein
Mammalian metallothioneins (MTs) are a class of low-molecular weight proteins containing 20 cysteine residues out of a total of 61 amino acid residues. MTs fold into two separate domains in the presence of cer...
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Chapter
The Improvement of Heavy Metal Tolerance in Cyanobacteria by Gene Engineering
Heavy metal pollution has been become the most serious in irrigated area of China with waste water, occupying about 64% the area[1], These heavy metals, such as cadmium(Cd), mercury(Hg) and lead(Pb), can enter in...
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Article
The complexation of apo-metallothionein with cadmium ion and its conformation study
The circular dichroism was used to study the complexation of two isoforms of rabbit liver apo-metallothioneins (apo-MT1 and apo-MT2) with Cd2+ ion and the influence of Cd2+ ion on the conformation of reconstitute...
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Article
α-Domain of human metallothionein IA can bind to metals in transgenic tobacco plants
With a view to exploring its use as a metal-binding factor in transgenic plants we prepared the α-domain of metallothionein by reconstitution of rabbit apometallothionein and proteolysis of MT-1 and MT-2 with ...
